Selective activation of phospholipase D-2 by unsaturated fatty acid
SCIE
SCOPUS
- Title
- Selective activation of phospholipase D-2 by unsaturated fatty acid
- Authors
- Kim, JH; Kim, Y; Lee, SD; Lopez, I; Arnold, RS; Lambeth, JD; Suh, PG; Ryu, SH
- Date Issued
- 1999-07-02
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate-stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 mu M in vitro. Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 mu M. These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2, (C) 1999 Federation of European Biochemical Societies.
- Keywords
- phospholipase D2; oleic acid; phosphatidylinositol 4,5-bisphosphate; ADP-RIBOSYLATION FACTOR; KINASE-C-ALPHA; RAT-BRAIN; PROTEIN-KINASE; ARACHIDONIC-ACID; PHOSPHATIDYLCHOLINE; D1; ISOFORM; ENZYMES; CLONING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20361
- DOI
- 10.1016/S0014-5793(99)00745-0
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS LETTERS, vol. 454, no. 1-2, page. 42 - 46, 1999-07-02
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- There are no files associated with this item.
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