Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M-3 muscarinic receptor
SCIE
SCOPUS
- Title
- Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M-3 muscarinic receptor
- Authors
- Kim, JY; Yang, MS; Oh, CD; Kim, KT; Ha, MJ; Kang, SS; Chun, JS
- Date Issued
- 1999-01-15
- Publisher
- PORTLAND PRESS
- Abstract
- The signalling pathway leading to an activation of mitogenactivated protein (MAP) kinase subtypes Erk-1 and -2 upon stimulation of muscarinic receptor with carbachol in human neuroblastoma SK-N-BE2(C) cells was investigated. Carbachol activated Erk-1/-2 by stimulating M-3 muscarinic receptor, as determined by specific antagonists for individual muscarinic receptors. The activation of Erk-1/-2 by carbachol was blocked by the inhibition or down-regulation of protein kinase C (PKC). Among the multiple PKC isoforms expressed in SK-N-BE2(C) cells, only PKC epsilon was activated by the treatment of carbachol, and selective down-regulation of PKC epsilon was sufficient to block Erk-l/-2 activation. Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Ectopic expression of inhibitory small GTPase Ras, RasN17, blocked the carbachol-induced Raf activation without affecting the activation of PKC epsilon, while the inhibition of PKC blocked the Raf activation. Thus, these results suggest that carbachol-induced activation of PKC epsilon mediates Erk 1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase.
- Keywords
- carbachol; mitogen-activated protein (MAP) kinase; protein kinase C; Raf; Pas; ACETYLCHOLINE-RECEPTOR; CELLULAR-RESPONSES; SIGNALING PATHWAY; ADENYLYL-CYCLASE; C ISOZYMES; CELLS; INHIBITOR; MAP; RAF; INVOLVEMENT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20524
- DOI
- 10.1042/0264-6021:3370275
- ISSN
- 0264-6021
- Article Type
- Article
- Citation
- BIOCHEMICAL JOURNAL, vol. 337, page. 275 - 280, 1999-01-15
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