Partial recovery of cell membrane-bounded human interleukin-2 fusion protein from insect cell debris by using various detergent extractions
SCIE
SCOPUS
- Title
- Partial recovery of cell membrane-bounded human interleukin-2 fusion protein from insect cell debris by using various detergent extractions
- Authors
- Cho, HS; Shin, HS; Bentley, WE; Cha, HJ
- Date Issued
- 2001-12
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- We previously found that the human interleukin-2 (hIL-2) fused with green fluorescent protein (GFP) mainly remained in the insect cell debris after disruption due to the highly hydrophobic property of hIL-2 itself. Even though the significant GFPuv/hIL-2 fusion proteins were associated with cell membrane fractions, these were still functionally active. Therefore, to increase the total product yield, we performed partial recovery of the cell membrane-bounded hIL-2 fusion protein from the insoluble cell debris using several non-ionic, zwitterionic, and anionic detergents.
- Keywords
- detergent; green fluorescent protein; human interleukin-2; insect Tricoplusia ni larvae; membrane protein; GREEN FLUORESCENT PROTEIN; RECOMBINANT HUMAN INTERLEUKIN-2; ESCHERICHIA-COLI; EXPRESSION; PURIFICATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20939
- DOI
- 10.1023/A:1013782231455
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 23, no. 23, page. 1957 - 1961, 2001-12
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.