DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, HD | - |
dc.contributor.author | Suh, BC | - |
dc.contributor.author | Joh, TH | - |
dc.contributor.author | Kim, KT | - |
dc.date.accessioned | 2016-03-31T14:23:25Z | - |
dc.date.available | 2016-03-31T14:23:25Z | - |
dc.date.created | 2009-03-18 | - |
dc.date.issued | 1996-03 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.other | 1996-OAK-0000009338 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21635 | - |
dc.description.abstract | We investigated transcriptional regulation of the rat tyrosine hydroxylase (TH) gene by muscarinic stimulation in human neuroblastoma SK-N-BE(2)M17 cells. Carbachol treatment increased the levels of intracellular Ca2+ and inositol 1,4,5-trisphosphate (IP3) and enhanced transcription of the TH gene, The muscarinic receptor antagonist atropine completely abolished the carbachol effect on TH gene expression. When cells were loaded with 50 mu M 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester (BAPTA/AM) to chelate intracellular Ca2+, carbachol still raised intracellular IP3 level and enhanced TH gene expression. Transient transfection analysis of the 5' upstream region of TH gene revealed that the AP1 cis-acting element at -205 to -199 bp was responsible for carbachol stimulation. But carbachol did not enhance TH gene expression in protein kinase C (PKC)-activated or downregulated cells that had been induced by 5-min or 24-h exposure to phorbol 12-myristate 13-acetate (PMA), respectively. Thus, Ca2+-independent PKC may play a role in carbachol-induced TH gene expression. We demonstrated by gel retardation and competition assays that a DNA sequence containing the wild-type AP1 site formed the specific DNA-protein complex. However, treatment with carbachol or PMA did not change the amount of the specific DNA-protein complex. Our results indicate that stimulation of phospholipase C-linked muscarinic receptors leads to elevated TH gene expression via AP1-mediated enhancement in a PKC-dependent pathway. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | LIPPINCOTT-RAVEN PUBL | - |
dc.relation.isPartOf | JOURNAL OF NEUROCHEMISTRY | - |
dc.subject | tyrosine hydroxylase | - |
dc.subject | muscarinic receptor | - |
dc.subject | AP1 | - |
dc.subject | protein kinase C | - |
dc.subject | PROTEIN-KINASE-C | - |
dc.subject | SH-SY5Y NEUROBLASTOMA-CELLS | - |
dc.subject | PARALLEL UP-REGULATION | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | CHLORAMPHENICOL ACETYLTRANSFERASE | - |
dc.subject | MEMBRANE DEPOLARIZATION | - |
dc.subject | RECEPTOR SUBTYPES | - |
dc.subject | ADRENAL-GLAND | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | CLONED GENES | - |
dc.title | AP1-mediated transcriptional enhancement of the rat: Tyrosine hydroxylase gene by muscarinic stimulation | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1046/j.1471-4159.1996.66031264.x | - |
dc.author.google | Chae, HD | - |
dc.author.google | Suh, BC | - |
dc.author.google | Joh, TH | - |
dc.author.google | Kim, KT | - |
dc.relation.volume | 66 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 1264 | - |
dc.relation.lastpage | 1272 | - |
dc.contributor.id | 10104775 | - |
dc.relation.journal | JOURNAL OF NEUROCHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, v.66, no.3, pp.1264 - 1272 | - |
dc.identifier.wosid | A1996TV78600048 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 1272 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1264 | - |
dc.citation.title | JOURNAL OF NEUROCHEMISTRY | - |
dc.citation.volume | 66 | - |
dc.contributor.affiliatedAuthor | Kim, KT | - |
dc.identifier.scopusid | 2-s2.0-0030023053 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 15 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | SH-SY5Y NEUROBLASTOMA-CELLS | - |
dc.subject.keywordPlus | PARALLEL UP-REGULATION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | CHLORAMPHENICOL ACETYLTRANSFERASE | - |
dc.subject.keywordPlus | MEMBRANE DEPOLARIZATION | - |
dc.subject.keywordPlus | RECEPTOR SUBTYPES | - |
dc.subject.keywordPlus | ADRENAL-GLAND | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | CLONED GENES | - |
dc.subject.keywordAuthor | tyrosine hydroxylase | - |
dc.subject.keywordAuthor | muscarinic receptor | - |
dc.subject.keywordAuthor | AP1 | - |
dc.subject.keywordAuthor | protein kinase C | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
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