CLONING, SEQUENCING AND EXPRESSION OF THE GENE ENCODING A MAJOR ALLOTYPIC PREPROCARBOXYPEPTIDASE-A FROM BOVINE PANCREAS
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- Title
- CLONING, SEQUENCING AND EXPRESSION OF THE GENE ENCODING A MAJOR ALLOTYPIC PREPROCARBOXYPEPTIDASE-A FROM BOVINE PANCREAS
- Authors
- GOO, JH; KIM, KH; CHOI, KY
- Date Issued
- 1995-11-20
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- A cDNA encoding one of two major allotypic forms of bovine pancreatic preprocarboxypeptidase A (preproCPA) has been cloned, and its entire nucleotide (nt) sequence determined. The cloned cDNA contains a 26-nt T-noncoding region, a 1260-nt open reading frame and a 51-nt 3'-noncoding region. The amino acid (aa) sequence deduced from the gene sequence contains Ile(179), Ala(228) and Val(305) at allotypic aa residues. The procarboxypeptidase A (proCPA) was produced in yeast and secreted into the medium. Upon treatment with trypsin, proCPA generated an active enzyme with the same size as the mature carboxypeptidase A (CPA), as analyzed by use of anti-CPA polyclonal antibody.
- Keywords
- CARBOXYPEPTIDASE A; SECRETION IN YEAST; ALLELOMORPHISM; POLYCLONAL ANTIBODY; METALLOEXOPEPTIDASE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21688
- DOI
- 10.1016/0378-1119(95)00454-E
- ISSN
- 0378-1119
- Article Type
- Article
- Citation
- GENE, vol. 165, no. 2, page. 333 - 334, 1995-11-20
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- There are no files associated with this item.
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