EXTENDED PATH-LENGTH POSTCOLUMN FLOW CELL FOR UV-VISIBLE ABSORBENCY DETECTION IN CAPILLARY ELECTROPHORESIS
SCIE
SCOPUS
- Title
- EXTENDED PATH-LENGTH POSTCOLUMN FLOW CELL FOR UV-VISIBLE ABSORBENCY DETECTION IN CAPILLARY ELECTROPHORESIS
- Authors
- KIM, S; KIM, W; HAHN, JH
- Date Issued
- 1994-09-30
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- A new type of post-column flow cell with extended optical path length has been developed to improve sensitivity of UV-visible absorbance detection for capillary electrophoresis. In this flow cell, the outlet of the capillary column is connected vertically to the middle of a 3-mm microchannel. Auxiliary flows are employed to flush substances eluted from the outlets of the microchannel. The optical path length is the full length of the microchannel. An 8-fold signal-to-noise ratio improvement over on-column detection has been demonstrated. However, the postcolumn flow cell suffered 44% loss in resolution. A limit of detection of 3.2 10(-7) M has been obtained for fluorescein isothiocyanate.
- Keywords
- ZONE ELECTROPHORESIS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21909
- DOI
- 10.1016/0021-9673(94)80058-8
- ISSN
- 0021-9673
- Article Type
- Article
- Citation
- JOURNAL OF CHROMATOGRAPHY A, vol. 680, no. 1, page. 109 - 116, 1994-09-30
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- There are no files associated with this item.
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