DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, SG | - |
dc.contributor.author | Lee, CS | - |
dc.contributor.author | Hwang, K | - |
dc.contributor.author | Ahn, JH | - |
dc.contributor.author | Hwang, S | - |
dc.date.accessioned | 2016-04-01T01:07:59Z | - |
dc.date.available | 2016-04-01T01:07:59Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.other | 2008-OAK-0000008227 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/22451 | - |
dc.description.abstract | The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders-Methanobacteriales, Methanomicrobiales, and Methanosarcinales-throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.relation.isPartOf | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | Diversity of methanogens | - |
dc.subject | Taxonomic level | - |
dc.subject | Order-specific primers | - |
dc.subject | Real-time quantitative PCR | - |
dc.subject | Denaturing gradient gel electrophoresis | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | 16S RIBOSOMAL-RNA | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | GROUP-SPECIFIC QUANTIFICATION | - |
dc.subject | GRADIENT GEL-ELECTROPHORESIS | - |
dc.subject | MICROBIAL COMMUNITY | - |
dc.subject | START-UP | - |
dc.subject | ANAEROBIC BIOREACTOR | - |
dc.subject | POPULATION-DYNAMICS | - |
dc.subject | PROBE SETS | - |
dc.title | Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system | - |
dc.type | Article | - |
dc.contributor.college | 환경공학부 | - |
dc.identifier.doi | 10.1007/s10295-008-0417-7 | - |
dc.author.google | Shin, SG | - |
dc.author.google | Lee, CS | - |
dc.author.google | Hwang, K | - |
dc.author.google | Ahn, JH | - |
dc.author.google | Hwang, S | - |
dc.relation.volume | 35 | - |
dc.relation.issue | 11 | - |
dc.relation.startpage | 1345 | - |
dc.relation.lastpage | 1352 | - |
dc.contributor.id | 10056523 | - |
dc.relation.journal | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Conference Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.35, no.11, pp.1345 - 1352 | - |
dc.identifier.wosid | 000260009200021 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1352 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1345 | - |
dc.citation.title | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Hwang, S | - |
dc.identifier.scopusid | 2-s2.0-54049126447 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 13 | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordPlus | GRADIENT GEL-ELECTROPHORESIS | - |
dc.subject.keywordPlus | GROUP-SPECIFIC QUANTIFICATION | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | MICROBIAL COMMUNITY | - |
dc.subject.keywordPlus | START-UP | - |
dc.subject.keywordPlus | ANAEROBIC BIOREACTOR | - |
dc.subject.keywordPlus | POPULATION-DYNAMICS | - |
dc.subject.keywordPlus | PROBE SETS | - |
dc.subject.keywordPlus | POLYMERASE | - |
dc.subject.keywordPlus | MICROFLORA | - |
dc.subject.keywordAuthor | Diversity of methanogens | - |
dc.subject.keywordAuthor | Taxonomic level | - |
dc.subject.keywordAuthor | Order-specific primers | - |
dc.subject.keywordAuthor | Real-time quantitative PCR | - |
dc.subject.keywordAuthor | Denaturing gradient gel electrophoresis | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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