A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion
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- Title
- A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion
- Authors
- Chung, KM; Cha, SS; Jang, SK
- Date Issued
- 2008-09-30
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOL
- Abstract
- Human karyopherin beta 3, highly homologous to a yeast protein secretion enhancer (PSE1), has often been reported to be associated with a mediator of a nucleocytoplasmic transport pathway. Previously, we showed that karyopherin beta 3 complemented the PSE1 and KAP123 double mutant. Our research suggested that karyopherin beta 3 has an evolutionary function similar to that of yeast PSE1 and/or KAP 123. In this study, we performed yeast two-hybrid screening to find a protein which would interact with karyopherin beta 3 and identified apolipoprotein A-I (apo A-I), a secretion protein with a primary function in cholesterol transport. By using in vitro binding assay, coimmunoprecipitation, and colocalization studies, we defined an interaction between karyopherin beta 3 and apo A-I. In addition, overexpression of karyopherin beta 3 significantly increased apo A-I secretion. These results suggest that karyopherin beta 3 plays a crucial role in apo A-I secretion. These findings may be relevant to the study of a novel function of karyopherin beta 3 and coronary artery diseases associated with apo A-I.
- Keywords
- apolipoprotein A-I; coronary artery diseases; karyopherin beta 3; PSE1; secretion enhancer; NUCLEAR IMPORT RECEPTORS; MINOR CAPSID PROTEIN; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; RIBOSOMAL-PROTEINS; CHOLESTEROL EFFLUX; MAMMALIAN-CELLS; PORE COMPLEX; TRANSPORT; INTERACTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22474
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 26, no. 3, page. 291 - 298, 2008-09-30
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