Label-free detection of antibody-antigen interactions on (R)-lipo-diaza-18-crown-6 self-assembled monolayer modified gold electrodes
SCIE
SCOPUS
- Title
- Label-free detection of antibody-antigen interactions on (R)-lipo-diaza-18-crown-6 self-assembled monolayer modified gold electrodes
- Authors
- Park, JY; Lee, YS; Kim, BH; Park, SM
- Date Issued
- 2008-07-01
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Novel (R)-diaza-18-crown-6 has been prepared by a simple two-step synthetic method and characterized for its ability to form a uniform self-assembled monolayer (SAM) on gold as well as to immobilize proteins using atomic force microscopy, quartz crystal microbalance, and electrochemical impedance spectroscopy (EIS) experiments. The (R)-lipo-diaza-18-crown-6 was shown to form a well-defined SAM on gold, which subsequently captures the antibody (Ab) molecules that in turn capture the antigen (Ag) molecules. The Ab molecules studied include antibody Greactive protein (Ab-CRP) and antibody ferritin (Ab-ferritin) along with their Ag's, i.e., CRP and ferritin. Quantitative detection of the Ab-Ag interactions was accomplished by EIS experiments with a Fe(CN)(6)(3-/4-) redox probe present. The ratios of the charge-transfer resistances for the redox probe on the SAM-antibody-covered electrode to those with the antigen molecules attached show an excellent linearity for log[Ag] with lower detection limits than those of other SAMs for the electrochemical sensing of proteins.
- Keywords
- C-REACTIVE PROTEIN; SURFACE-PLASMON RESONANCE; ALPHA-FETOPROTEIN; IMMUNOASSAY; NANOPARTICLES; MOLECULES; MARKER; ELISA; IMMOBILIZATION; INFLAMMATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/22672
- DOI
- 10.1021/AC8002374
- ISSN
- 0003-2700
- Article Type
- Article
- Citation
- ANALYTICAL CHEMISTRY, vol. 80, no. 13, page. 4986 - 4993, 2008-07-01
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