Ligand profiling and identification technology for searching bioactive ligands
SCIE
SCOPUS
- Title
- Ligand profiling and identification technology for searching bioactive ligands
- Authors
- Baek, MC; Kim, SJ; Yea, K; Kim, Y; Lee, BD; Kim, J; Lee, HJ; Kang, MH; Choi, SK; Kim, JI; Lee, TG; Suh, PG; Ryu, SH
- Date Issued
- 2006-03
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.
- Keywords
- identification; ligand; peptidome; profiling; HUMAN INSULIN-RECEPTOR; PROTEIN-COUPLED RECEPTORS; HUMAN-GENOME-PROJECT; MASS-SPECTROMETRY; CLEAVAGE SITES; KINASE; DIARGINYLINSULIN; PROINSULIN; PROTEOMICS; PEPTIDE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24113
- DOI
- 10.1002/pmic.200500511
- ISSN
- 1615-9853
- Article Type
- Article
- Citation
- PROTEOMICS, vol. 6, no. 6, page. 1741 - 1749, 2006-03
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- There are no files associated with this item.
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