MOSFET-type biosensor for detection of streptavidin-biotin protein complexes
SCIE
SCOPUS
- Title
- MOSFET-type biosensor for detection of streptavidin-biotin protein complexes
- Authors
- Kim, DS; Park, HJ; Park, JE; Shin, JK; Kang, SW; Seo, HI; Lim, G
- Date Issued
- 2005-01
- Publisher
- MYU K K
- Abstract
- A p-channel metal oxide semiconductor field-effect transistor (PMOSFET)-type biosensor for detecting streptavidin-biotin protein complexes has been fabricated. Au, which has a chemical affinity with thiol, was used as the gate metal in order to form a self-assembled monolayer (SAM). A SAM was used to immobilize streptavidin. The hydroxyl group of SAM was bound with the amine group of streptavidin. Biotin was then injected into the solution to form streptavidin-biotin protein complexes in the solution. Streptavidin and biotin were bound by a high affinity (Ka similar to 10(15)Mol(-1)). Measurements were conducted in a phosphate buffer saline (PBS; pH 6.4, 20 mu M) solution and a Pt electrode was used as the reference electrode. The bindings of SAM, streptavidin, and biotin caused a variation in the drain current of the PMOSFET-type biosensor. To verify interactions among SAM, streptaividn, and biotin, a quartz crystal microbalance QCM) measurement was performed.
- Keywords
- FET-type sensor; biosensor; protein detection; self-assembled monolayer (SAM); SELF-ASSEMBLED MONOLAYERS; IMMOBILIZATION; GOLD
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24454
- ISSN
- 0914-4935
- Article Type
- Article
- Citation
- SENSORS AND MATERIALS, vol. 17, no. 5, page. 259 - 268, 2005-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.