Microinjection Molded Disposable Microfluidic Lab-on-a-Chip for Efficient Detection of Agglutination
SCIE
SCOPUS
- Title
- Microinjection Molded Disposable Microfluidic Lab-on-a-Chip for Efficient Detection of Agglutination
- Authors
- Choi, SH; Kim, DS; Tai Hun Kwon
- Date Issued
- 2009-02
- Publisher
- SPRINGER
- Abstract
- Previous diagnosing methods based on agglutination have a limitation in view of emergency and point-of-care diagnoses due to the requirement of large scale equipments and much agglutination time. In this paper, we propose a low cost microfluidic lab-on-a-chip for more efficient detection of agglutination. In the present lab-on-a-chip, two inlet microwells, flow guiding microchannels, chaotic micromixer and reaction microwell are fully integrated. Mold inserts for the lab-on-a-chip were manufactured by UV photolithography and nickel electroplating process. The complete lab-on-a-chip was realized by the microinjection molding of cyclic olefin copolymer and the subsequent thermal bonding. The improved serpentine laminating micromixer, developed by our group, integrated in the lab-on-a-chip showed the high-level of chaotic mixing, thereby enabling us to get a reliable mixing of sample and reagent. The performance of the fabricated lab-on-a-chip was demonstrated by agglutination experiments with simulated bloods of 10 mu l and simulated sera of 10 mu l. The results of agglutination inside the reaction microwell were clearly read by means of the level of light transmission. The present microfluidic lab-on-a-chip could be widely applied to various clinical diagnostics based on agglutination tests.
- Keywords
- SERPENTINE LAMINATING MICROMIXER; TOTAL ANALYSIS SYSTEMS; ADVECTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25022
- DOI
- 10.1007/S00542-008-0689-X
- ISSN
- 0946-7076
- Article Type
- Article
- Citation
- MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS, vol. 15, no. 2, page. 309 - 316, 2009-02
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