DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, SW | - |
dc.contributor.author | Kim, JS | - |
dc.contributor.author | Park, KS | - |
dc.contributor.author | Cha, BH | - |
dc.contributor.author | Shim, JH | - |
dc.contributor.author | Kim, JY | - |
dc.contributor.author | Cho, DW | - |
dc.contributor.author | Rhie, JW | - |
dc.contributor.author | Lee, SH | - |
dc.date.accessioned | 2016-04-01T02:23:24Z | - |
dc.date.available | 2016-04-01T02:23:24Z | - |
dc.date.created | 2011-03-11 | - |
dc.date.issued | 2011-02-01 | - |
dc.identifier.issn | 8756-3282 | - |
dc.identifier.other | 2011-OAK-0000022890 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25042 | - |
dc.description.abstract | Bone tissue engineering often requires a well-defined scaffold that is highly porous. The multi-head deposition system (MHDS), a form of solid freeform fabrication, has raised great interest as a method for fabricating scaffolds, since it yields a highly porous inter-connective structure without the use of cytotoxic solvents, and permits the diffusion of nutrients and oxygen. However, this method is not suitable for introducing proteins, as it includes a heating process. Hydrogels incorporated with protein coating of the scaffold surface could overcome this MHDS limitation. In the present study, the surface of a scaffold fabricated using MHDS was coated with a mixture of fibrin and hyaluronic acid (HA) and used as a vehicle for delivery of both bone morphogenetic protein-2 (BMP-2) and adipose-derived stromal cells (ASCs). Fibrin/HA coating of the scaffold significantly enhanced initial cell attachment. Furthermore, the in vitro release of BMP-2 from fibrin/HA-coated scaffolds was sustained for 3 days and it stimulated the alkaline phosphatase activity of ASCs seeded on the scaffold for 10 days more actively and continuously than did the soluble BMP-2 that was added to the culture media, not the scaffold itself. Importantly, the transplantation of undifferentiated ASCs inoculated on BMP-2-loaded, fibrin/HA-coated scaffolds resulted in more improved bone formation and mineralization than did the transplantation of undifferentiated ASCs seeded on uncoated scaffolds or on fibrin/HA-coated scaffolds without BMP-2, but containing BMP-2 in the cell suspension medium. These results show that BMP-2-loaded, fibrin/HA-coated scaffolds fabricated using MHDS may be useful in stimulating bone regeneration from undifferentiated ASCs in vivo. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | BONE | - |
dc.subject | BMP | - |
dc.subject | Bone tissue engineering | - |
dc.subject | Fibrin | - |
dc.subject | Hyaluronic acid | - |
dc.subject | Surface modification | - |
dc.subject | MESENCHYMAL STEM-CELLS | - |
dc.subject | HEAD DEPOSITION SYSTEM | - |
dc.subject | MORPHOGENETIC PROTEIN-2 | - |
dc.subject | FABRICATION TECHNIQUES | - |
dc.subject | POROUS HYDROXYAPATITE | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | DESIGN | - |
dc.subject | GROWTH | - |
dc.subject | RATIONALE | - |
dc.subject | POLYMERS | - |
dc.title | Surface modification with fibrin/hyaluronic acid hydrogel on solid-free form-based scaffolds followed by BMP-2 loading to enhance bone regeneration | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1016/J.BONE.2010.09.029 | - |
dc.author.google | Kong, SW | - |
dc.author.google | Kim, JS | - |
dc.author.google | Park, KS | - |
dc.author.google | Cha, BH | - |
dc.author.google | Shim, JH | - |
dc.author.google | Kim, JY | - |
dc.author.google | Cho, DW | - |
dc.author.google | Rhie, JW | - |
dc.author.google | Lee, SH | - |
dc.relation.volume | 48 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 298 | - |
dc.relation.lastpage | 306 | - |
dc.contributor.id | 10102903 | - |
dc.relation.journal | BONE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BONE, v.48, no.2, pp.298 - 306 | - |
dc.identifier.wosid | 000286543700017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 306 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 298 | - |
dc.citation.title | BONE | - |
dc.citation.volume | 48 | - |
dc.contributor.affiliatedAuthor | Cho, DW | - |
dc.identifier.scopusid | 2-s2.0-78650976404 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 76 | - |
dc.description.scptc | 71 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | HEAD DEPOSITION SYSTEM | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | FABRICATION TECHNIQUES | - |
dc.subject.keywordPlus | POROUS HYDROXYAPATITE | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | RATIONALE | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordAuthor | BMP | - |
dc.subject.keywordAuthor | Bone tissue engineering | - |
dc.subject.keywordAuthor | Fibrin | - |
dc.subject.keywordAuthor | Hyaluronic acid | - |
dc.subject.keywordAuthor | Surface modification | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
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