DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, H | - |
dc.contributor.author | Min, K | - |
dc.contributor.author | Song, KM | - |
dc.contributor.author | Ku, JK | - |
dc.contributor.author | Han, MS | - |
dc.contributor.author | Ban, C | - |
dc.date.accessioned | 2016-04-01T02:25:05Z | - |
dc.date.available | 2016-04-01T02:25:05Z | - |
dc.date.created | 2011-03-09 | - |
dc.date.issued | 2011-01-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.other | 2011-OAK-0000022801 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25093 | - |
dc.description.abstract | We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature (T-m). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.subject | Screening | - |
dc.subject | dsDNA binder | - |
dc.subject | Magnetic bead | - |
dc.subject | Hairpin DNA | - |
dc.subject | HPLC | - |
dc.subject | ANTICANCER DRUG SCREEN | - |
dc.subject | SMALL-MOLECULE | - |
dc.subject | COMBINATORIAL APPROACH | - |
dc.subject | NANOPARTICLE PROBES | - |
dc.subject | CANCER-CELLS | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | BINDING | - |
dc.subject | AGENTS | - |
dc.subject | DOXORUBICIN | - |
dc.subject | COMPLEXES | - |
dc.title | Simple Screening Method for Double-strand DNA Binders Using Hairpin DNA-modified Magnetic Beads | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.5012/BKCS.2011.32.1.247 | - |
dc.author.google | Jo, H | - |
dc.author.google | Min, K | - |
dc.author.google | Song, KM | - |
dc.author.google | Ku, JK | - |
dc.author.google | Han, MS | - |
dc.author.google | Ban, C | - |
dc.relation.volume | 32 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 247 | - |
dc.relation.lastpage | 250 | - |
dc.contributor.id | 10085220 | - |
dc.relation.journal | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.1, pp.247 - 250 | - |
dc.identifier.wosid | 000286538600045 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 250 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 247 | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Ku, JK | - |
dc.contributor.affiliatedAuthor | Ban, C | - |
dc.identifier.scopusid | 2-s2.0-78951481721 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 1 | - |
dc.description.scptc | 1 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SMALL-MOLECULE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | AGENTS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | ETHIDIUM | - |
dc.subject.keywordAuthor | Screening | - |
dc.subject.keywordAuthor | dsDNA binder | - |
dc.subject.keywordAuthor | Magnetic bead | - |
dc.subject.keywordAuthor | Hairpin DNA | - |
dc.subject.keywordAuthor | HPLC | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
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