Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment
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- Title
- Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment
- Authors
- Yoon, J; Kim, ES; Lee, SJ; Park, CW; Cha, HJ; Hong, BH; Choi, KY
- Date Issued
- 2010-12
- Publisher
- KOREAN SOC GYNECOLOGY ONCOLOGY & COLPOSCOPY
- Abstract
- Objective: The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment. Methods: We used IC(50) values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray. Results: The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3. Conclusion: In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
- Keywords
- Ovarian cancer cell lines; Cisplatin; Apoptosis; Dendron-modified DNA microarray; CDNA MICROARRAY; TUMOR-CELLS; DNA-REPAIR; RESISTANCE; IDENTIFICATION; ACCUMULATION; CARCINOMA; RESPONSES; PROTEINS; DEATH
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25123
- DOI
- 10.3802/JGO.2010.21.4.255
- ISSN
- 2005-0380
- Article Type
- Article
- Citation
- JOURNAL OF GYNECOLOGIC ONCOLOGY, vol. 21, no. 4, page. 255 - 261, 2010-12
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