DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dong, CH | - |
dc.contributor.author | Jang, M | - |
dc.contributor.author | Scharein, B | - |
dc.contributor.author | Malach, A | - |
dc.contributor.author | Rivarola, M | - |
dc.contributor.author | Liesch, J | - |
dc.contributor.author | Groth, G | - |
dc.contributor.author | Hwang, I | - |
dc.contributor.author | Chang, C | - |
dc.date.accessioned | 2016-04-01T02:27:03Z | - |
dc.date.available | 2016-04-01T02:27:03Z | - |
dc.date.created | 2011-02-07 | - |
dc.date.issued | 2010-12-24 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2011-OAK-0000022670 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25155 | - |
dc.description.abstract | The plant hormone ethylene plays important roles in growth and development. Ethylene is perceived by a family of membrane-bound receptors that actively repress ethylene responses. When the receptors bind ethylene, their signaling is shut off, activating responses. REVERSION-TO-ETHYLENE SENSITIVITY (RTE1) encodes a novel membrane protein conserved in plants and metazoans. Genetic analyses in Arabidopsis thaliana suggest that RTE1 promotes the signaling state of the ethylene receptor ETR1 through the ETR1 N-terminal domain. RTE1 and ETR1 have been shown to co-localize to the endoplasmic reticulum (ER) and Golgi apparatus in Arabidopsis. Here, we demonstrate a physical association of RTE1 and ETR1 using in vivo and in vitro methods. Interaction of RTE1 and ETR1 was revealed in vivo by bimolecular fluorescence complementation (BiFC) in a tobacco cell transient assay and in stably transformed Arabidopsis. The association was also observed using a truncated version of ETR1 comprising the N terminus (amino acids 1-349). Interaction of RTE1 and ETR1 was confirmed by co-immunoprecipitation from Arabidopsis. The interaction occurs with high affinity (K-d, 117 nM) based on tryptophan fluorescence spectroscopy using purified recombinant RTE1 and a tryptophan-less version of purified recombinant ETR1. An amino acid substitution (C161Y) in RTE1 that is known to confer an ETR1 loss-of-function phenotype correspondingly gives a nearly 12-fold increase in the dissociation constant (K-d, 1.38 mu M). These findings indicate that a high affinity association of RTE1 and ETR1 is important in the regulation of ETR1. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | BIMOLECULAR FLUORESCENCE COMPLEMENTATION | - |
dc.subject | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject | HISTIDINE KINASE-ACTIVITY | - |
dc.subject | SUBCELLULAR-LOCALIZATION | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | FAMILY-MEMBERS | - |
dc.subject | GENE FAMILY | - |
dc.subject | THALIANA | - |
dc.subject | BINDING | - |
dc.title | Molecular Association of the Arabidopsis ETR1 Ethylene Receptor and a Regulator of Ethylene Signaling, RTE1 | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1074/JBC.M110.146605 | - |
dc.author.google | Dong, CH | - |
dc.author.google | Jang, M | - |
dc.author.google | Scharein, B | - |
dc.author.google | Malach, A | - |
dc.author.google | Rivarola, M | - |
dc.author.google | Liesch, J | - |
dc.author.google | Groth, G | - |
dc.author.google | Hwang, I | - |
dc.author.google | Chang, C | - |
dc.relation.volume | 285 | - |
dc.relation.issue | 52 | - |
dc.relation.startpage | 40706 | - |
dc.relation.lastpage | 40713 | - |
dc.contributor.id | 10078446 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.285, no.52, pp.40706 - 40713 | - |
dc.identifier.wosid | 000285414400034 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 40713 | - |
dc.citation.number | 52 | - |
dc.citation.startPage | 40706 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 285 | - |
dc.contributor.affiliatedAuthor | Hwang, I | - |
dc.identifier.scopusid | 2-s2.0-78650364951 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 54 | - |
dc.description.scptc | 58 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BIMOLECULAR FLUORESCENCE COMPLEMENTATION | - |
dc.subject.keywordPlus | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | HISTIDINE KINASE-ACTIVITY | - |
dc.subject.keywordPlus | SUBCELLULAR-LOCALIZATION | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FAMILY-MEMBERS | - |
dc.subject.keywordPlus | GENE FAMILY | - |
dc.subject.keywordPlus | THALIANA | - |
dc.subject.keywordPlus | BINDING | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.