Mineralization and transformation of monofluorophenols by Pseudonocardia benzenivorans
SCIE
SCOPUS
- Title
- Mineralization and transformation of monofluorophenols by Pseudonocardia benzenivorans
- Authors
- Kim, EJ; Jeon, JR; Kim, YM; Murugesan, K; Chang, YS
- Date Issued
- 2010-07
- Publisher
- SPRINGER
- Abstract
- The aerobic metabolism of monofluorophenols (mono-FPs) by the actinomycete, Pseudonocardia benzenivorans, was studied. This strain was able to grow on 4-fluorophenol (4-FP) and readily transform 2- and 3-fluorophenol to the corresponding metabolites. The detailed mechanism of mono-FPs degradation by P. benzenivorans was elucidated from enzymatic assays and the identification of reaction intermediates by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry. Two types of fluorocatechols (i.e., 3- and 4-fluorocatechol) were identified as the key transformation products. During 4-FP degradation, only 4-fluorocatechol was detected, and a stoichiometric level of fluoride was released. Both fluorocatechols were observed together in cultures containing 3-fluorophenol (3-FP), while only 3-fluorocatechol was found to accumulate in 2-fluorophenol (2-FP)-containing cultures. Whole-cell extracts of P. benzenivorans expressed catechol 1,2-dioxygenase activity, indicating that the transformation of the three tested mono-FPs proceeded via ortho-cleavage pathway. The results presented in this paper provide comprehensive information regarding the metabolism of mono-FPs by a single bacterium.
- Keywords
- Biodegradation; Monofluorophenols; 4-Fluorophenol; Pseudonocardia benzenivorans; RHODOCOCCUS-OPACUS; MICROBIAL-DEGRADATION; BACTERIAL-DEGRADATION; 6-FLUORO SUBSTITUENT; AROMATIC-COMPOUNDS; METABOLISM; CATECHOL; PHENOLS; BIODEGRADATION; DEHALOGENATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25403
- DOI
- 10.1007/S00253-010-2647-7
- ISSN
- 0175-7598
- Article Type
- Article
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 4, page. 1569 - 1577, 2010-07
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