DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, KH | - |
dc.contributor.author | SEO, JONGCHEOL | - |
dc.contributor.author | Yoon, HJ | - |
dc.contributor.author | Shin, SK | - |
dc.date.accessioned | 2016-04-01T02:38:40Z | - |
dc.date.available | 2016-04-01T02:38:40Z | - |
dc.date.created | 2010-12-02 | - |
dc.date.issued | 2010-08-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.other | 2010-OAK-0000022127 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25509 | - |
dc.description.abstract | Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while alpha-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive detection of peptides by homogeneously depositing matrix and sample under atmospheric pressure. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.title | Focused Electrospray Deposition for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.5012/BKCS.2010.31.8.2293 | - |
dc.author.google | Jeong, KH | - |
dc.author.google | Seo, J | - |
dc.author.google | Yoon, HJ | - |
dc.author.google | Shin, SK | - |
dc.relation.volume | 31 | - |
dc.relation.issue | 8 | - |
dc.relation.startpage | 2293 | - |
dc.relation.lastpage | 2298 | - |
dc.contributor.id | 10200277 | - |
dc.relation.journal | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.8, pp.2293 - 2298 | - |
dc.identifier.wosid | 000281700100033 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 2298 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2293 | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | SEO, JONGCHEOL | - |
dc.contributor.affiliatedAuthor | Shin, SK | - |
dc.identifier.scopusid | 2-s2.0-77956108268 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 1 | - |
dc.description.scptc | 1 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MALDI-MS ANALYSIS | - |
dc.subject.keywordPlus | ATMOSPHERIC-PRESSURE MALDI | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | 2,5-DIHYDROXYBENZOIC ACID | - |
dc.subject.keywordPlus | DESORPTION IONIZATION | - |
dc.subject.keywordPlus | PLASMA DESORPTION | - |
dc.subject.keywordPlus | FAST-EVAPORATION | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordAuthor | Focused electrospray | - |
dc.subject.keywordAuthor | Atmospheric pressure ion focusing | - |
dc.subject.keywordAuthor | Matrix-assisted laser desorption ionization | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
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