DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, S | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Yea, K | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Ryu, SH | - |
dc.date.accessioned | 2016-04-01T02:44:33Z | - |
dc.date.available | 2016-04-01T02:44:33Z | - |
dc.date.created | 2010-11-12 | - |
dc.date.issued | 2010-06-15 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.other | 2010-OAK-0000021822 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25685 | - |
dc.description.abstract | Adipocytes are well known to release regulation factors associated with metabolic disorders. In particular, increased oxidative stress in adipocytes contributes to dysregulation of adipokine production. In this study, we applied relative quantitative proteomic analysis based on label-free multiple reaction monitoring (MRM) to discover biological changes of adipokines under oxidative stress. Among a total of 194 identified proteins, 8 proteins were selected and quantified between control and hydrogen peroxide (H2O2)-treated groups by label-free MRM quantification. The secretion levels of matrix metalloproteinase-2 (MMP-2), stromal cell-derived factor-1 (SDF-1, CXCL12), resistin, and complement factor D (CFD, adipsin) decreased, whereas the secretion levels of tissue inhibitor of metalloproteinase-2 (TIMP-2) and aldolase A increased. Here we suggest that our study with label-free quantitative analysis will contribute to the efficient quantitative analysis of targeted proteins in complex mixtures and specifically to a better understanding of changes of adipokines under oxidative stress. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.subject | Label-free quantification | - |
dc.subject | Multiple reaction monitoring | - |
dc.subject | Triple quadrupole mass spectrometry | - |
dc.subject | Oxidative stress | - |
dc.subject | Adipocytes | - |
dc.subject | Adipokines | - |
dc.subject | INDUCED INSULIN-RESISTANCE | - |
dc.subject | SERINE PROTEASE HOMOLOG | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | ENDOCRINE ORGAN | - |
dc.subject | 3T3-L1 ADIPOCYTES | - |
dc.subject | QUANTIFICATION | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEOMICS | - |
dc.subject | OBESITY | - |
dc.subject | METALLOPROTEINASES | - |
dc.title | Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1016/J.AB.2010.03.006 | - |
dc.author.google | Choi, S | - |
dc.author.google | Kim, J | - |
dc.author.google | Yea, K | - |
dc.author.google | Suh, PG | - |
dc.author.google | Ryu, SH | - |
dc.relation.volume | 401 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 196 | - |
dc.relation.lastpage | 202 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | ANALYTICAL BIOCHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.401, no.2, pp.196 - 202 | - |
dc.identifier.wosid | 000277397400004 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 202 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 196 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 401 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-77952851155 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 16 | - |
dc.description.scptc | 17 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INDUCED INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | ENDOCRINE ORGAN | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | METALLOPROTEINASES | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordAuthor | Label-free quantification | - |
dc.subject.keywordAuthor | Multiple reaction monitoring | - |
dc.subject.keywordAuthor | Triple quadrupole mass spectrometry | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | Adipocytes | - |
dc.subject.keywordAuthor | Adipokines | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.