DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, MJ | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Kim, MY | - |
dc.contributor.author | Bae, YS | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Lee, TG | - |
dc.contributor.author | Kim, JH | - |
dc.date.accessioned | 2016-04-01T02:44:44Z | - |
dc.date.available | 2016-04-01T02:44:44Z | - |
dc.date.created | 2010-11-24 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.other | 2010-OAK-0000021810 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25690 | - |
dc.description.abstract | Human adipose tissue-derived mesenchymal stem cells (hASCs) are useful for regeneration of inflamed or injured tissues. To identify secreted hASC proteins during inflammation, hASCs were exposed to tumor necrosis factor-alpha (TNF-alpha) and conditioned media derived from hASCs were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified 187 individual proteins as secreted proteins (secretome) in hASC-conditioned media; 118 proteins were secreted at higher levels upon TNF-a treatment. The TNF-alpha-induced secretome included a variety of cytokines and chemokines such as interleukin-6 (IL-6), IL-8, chemokine (C-X-C motif) ligand 6, and monocyte chemotactic protein-1 (MCP-1). TNF-alpha also increased expression of various proteases including cathepsin L, matrix metalloproteases and protease inhibitors, and induced secretion of long pentraxin 3, a key inflammatory mediator implicated in innate immunity. TNF-alpha-conditioned media stimulated migration of human monocytes, which play a key role in inflammatory responses. This migration was abrogated by pretreatment with neutralizing anti-IL-6, anti-IL-8, and anti-MCP-1 antibodies, suggesting that IL-6, IL-8, and MCP-1 are involved in migration of monocytes. Taken together, these results suggest that TNF-alpha-induced secretome may play a pivotal role in inflammatory responses and that shotgun proteomic analysis will be useful for elucidation of the paracrine functions of mesenchymal stem cells. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | JOURNAL OF PROTEOME RESEARCH | - |
dc.subject | mesenchymal stem cells | - |
dc.subject | secretome | - |
dc.subject | TNF-alpha | - |
dc.subject | monocytes | - |
dc.subject | chemotaxis | - |
dc.subject | LONG PENTRAXIN PTX3 | - |
dc.subject | HUMAN BONE-MARROW | - |
dc.subject | STROMAL CELLS | - |
dc.subject | CYSTEINE CATHEPSINS | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | LABEL-FREE | - |
dc.subject | EXPRESSION | - |
dc.subject | DISEASE | - |
dc.subject | INTERLEUKIN-6 | - |
dc.subject | ACTIVATION | - |
dc.title | Proteomic Analysis of Tumor Necrosis Factor-alpha-Induced Secretome of Human Adipose Tissue-Derived Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1021/PR900898N | - |
dc.author.google | Lee, Mi Jeong | - |
dc.author.google | Kim, Jaeyoon | - |
dc.author.google | Kim, Min Young | - |
dc.author.google | Bae, Yoe-Sik | - |
dc.author.google | Ryu, Sung Ho | - |
dc.author.google | Lee, Taehoon G. | - |
dc.author.google | Kim, Jae Ho | - |
dc.relation.volume | 9 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 1754 | - |
dc.relation.lastpage | 1762 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | JOURNAL OF PROTEOME RESEARCH | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF PROTEOME RESEARCH, v.9, no.4, pp.1754 - 1762 | - |
dc.identifier.wosid | 000276215700012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1762 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1754 | - |
dc.citation.title | JOURNAL OF PROTEOME RESEARCH | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-77950660594 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 88 | - |
dc.description.scptc | 83 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LONG PENTRAXIN PTX3 | - |
dc.subject.keywordPlus | HUMAN BONE-MARROW | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | CYSTEINE CATHEPSINS | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | INTERLEUKIN-6 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | mesenchymal stem cells | - |
dc.subject.keywordAuthor | secretome | - |
dc.subject.keywordAuthor | TNF-alpha | - |
dc.subject.keywordAuthor | monocytes | - |
dc.subject.keywordAuthor | chemotaxis | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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