DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Sim Sung Bo | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2016-04-01T02:45:01Z | - |
dc.date.available | 2016-04-01T02:45:01Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2007-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 2007-OAK-0000021798 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25698 | - |
dc.description.abstract | Previously, we designed and constructed a hybrid of the mussel adhesive protein (MAP) fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5) terminus. Through various cell-adhesion analyses, we previously demonstrated that fp-151 has the potential to be used as a cell or tissue bioadhesive. In the present study, to improve the cell-adhesion properties of fp-151, we designed a new cell-adhesive protein, fp-151-RGD, which is a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identified at the cell-attachment site of fibronectin, at the C-terminus of fp-151. Although recombinant fp-151-RGD maintained the advantages associated with fp-151, such as a high production yield in Escherichia coli and simple purification, it showed superior spreading ability, which is important for cell proliferation under serum-free conditions, as well as better cell-adhesion ability compared with other commercially produced cell-adhesion materials such as poly-L-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp-151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPA adhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion of fibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required. (c) 2007 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | elsevier | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.title | Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide | - |
dc.type | Article | - |
dc.contributor.college | 해양대학원 | - |
dc.identifier.doi | 10.1016/J.BIOMATERIALS.2007.05.028 | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Sim, SB | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 28 | - |
dc.relation.issue | 28 | - |
dc.relation.startpage | 4039 | - |
dc.relation.lastpage | 4046 | - |
dc.contributor.id | 10167197 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.28, no.28, pp.4039 - 4046 | - |
dc.identifier.wosid | 000248992900003 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 4046 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 4039 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-34447279470 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 111 | - |
dc.description.scptc | 114 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | BYSSUS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | COLLAGEN | - |
dc.subject.keywordPlus | MGFP-5 | - |
dc.subject.keywordAuthor | cell adhesion material | - |
dc.subject.keywordAuthor | Mussel adhesive protein | - |
dc.subject.keywordAuthor | RGD peptide | - |
dc.subject.keywordAuthor | Cell recognition motif | - |
dc.subject.keywordAuthor | Fusion protein | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.