Carassius auratus-originated recombinant histone H1 c-terminal peptide as Gene delivery material
- Title
- Carassius auratus-originated recombinant histone H1 c-terminal peptide as Gene delivery material
- Authors
- Jung, HJ; Hwang, DS; De Wei, Q; Cha, HJ; 황동수
- Date Issued
- 2008-02
- Publisher
- Wiley
- Abstract
- The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research. Protein-based gene delivery including histone proteins has recently emerged as a powerful technique for non-viral DNA transfer. Histories are DNA-binding proteins that function in DNA packaging and protection. In particular, histone H1 is largely responsible for the stabilization of higher-order chromatin structures. Several studies have examined the use of full-length histone H1-mediated gene transfer, and a few studies have investigated the use of C-terminal histone H1 fragments as gene-transfer materials. Previously, we cloned a novel histone H1 cDNA from the goldfish Carassius auratus and found that a recombinant histone H1 C-terminal short peptide (H1C) of 61 amino acids has comparable DNA binding and protection functions as full-length histone H1. In the present work, we successfully expressed and purified soluble recombinant H1 C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. We confirmed its DNA-binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells (human 293T and mouse NIH/3T3) than the widely used agent lipofectamine. Therefore, we suggest that this novel goldfish-derived recombinant histone H1 C-terminal short peptide could be used as a peptide-based gene-transfer mediator.
- Keywords
- ESCHERICHIA-COLI; H1-MEDIATED TRANSFECTION; DNA; H1; EXPRESSION; PROTEIN; BINDING; DOMAIN; IDENTIFICATION; COMPLEXES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25699
- DOI
- 10.1021/BP070069B
- ISSN
- 8756-7938
- Article Type
- Conference
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.