DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D | - |
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Kang, DG | - |
dc.contributor.author | Kim, JYH | - |
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | 황동수 | - |
dc.date.accessioned | 2016-04-01T02:45:05Z | - |
dc.date.available | 2016-04-01T02:45:05Z | - |
dc.date.issued | 2008-06 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS | - |
dc.identifier.citation | v.24 | - |
dc.identifier.citation | no.3 | - |
dc.identifier.citation | pp.663-666 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.other | 2008-OAK-0000021795 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25700 | - |
dc.description.abstract | Mussel adhesive proteins (MAPs) have been considered as potential underwater and medical bioadhesives. Previously, we reported a functional expression of recombinant MAP hybrid fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5) terminus, with practical properties in Escherichia coli. In the present work, we introduced the Vitreoscilla hemoglobin (VHb) co-expression strategy to enhance the production levels of hybrid fp-151 since VHb has been successfully used for efficient oxygen utilization in several expression systems, including E. coli. In both batch-type flask and fed-batch-type bioreactor cultures, we found that co-expression of VHb conferred higher cell growth and hybrid fp-151 production. Its positive effects were significantly increased in high cell density bioreactor cultures as the microaerobic environment was more quickly and severely formed. We obtained a similar to 1.9-fold higher (similar to 1 g/L) production of MAP fp-151 from VHb co-expressing cells in fed-batch bioreactor cultures as compared to that from VHb non-expressing cells. Collectively and regardless of the culture type, VHb co-expression strategy was successful in enhancing the production of recombinant mussel adhesive proteins in the E. coli expression system. | - |
dc.description.statementofresponsibility | X | - |
dc.publisher | American Chemical Society | - |
dc.subject | CHONDROCYTE TRANSPLANTATION SURGERY | - |
dc.subject | INDUCIBLE PROMOTER SYSTEM | - |
dc.subject | INACTIVATED NAR OPERON | - |
dc.subject | FED-BATCH CULTIVATION | - |
dc.subject | MYTILUS-EDULIS-L | - |
dc.subject | POLYPHENOLIC PROTEINS | - |
dc.subject | EXPRESSION | - |
dc.subject | VITREOSCILLA | - |
dc.subject | GENE | - |
dc.title | Enhancement of mussel adhesive protein production in Escherichia coli by co-expression of bacterial hemoglobin | - |
dc.type | Conference | - |
dc.contributor.college | 해양대학원 | - |
dc.author.google | Kim, D | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Kang, DG | - |
dc.author.google | Kim, JYH | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 24 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 663 | - |
dc.relation.lastpage | 666 | - |
dc.contributor.id | 10167197 | - |
dc.publisher.location | US | - |
dc.relation.journal | BIOTECHNOLOGY PROGRESS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Conference Papers | - |
dc.type.docType | Conference Paper | - |
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