PRODUCTION OF FUSION MUSSEL ADHESIVE FP-353 IN ESCHERICHIA COLI
SCIE
SCOPUS
- Title
- PRODUCTION OF FUSION MUSSEL ADHESIVE FP-353 IN ESCHERICHIA COLI
- Authors
- Youngsoo Gim; Hwang, DS; Sunghae Lim; Younghoon Song; CHA, HYUNG JOON
- Date Issued
- 2008-11
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Mussel adhesive proteins (MAPs) have a potential as environmentally friendly adhesives for use under aqueous conditions. MAPs maybe of particular value in medical applications. We previously reported the functional expression of recombinant foot protein type 5 (fp-5) and foot protein type 3A (fp-3A), both of which have significant adhesion abilities, in Escherichia coli. However, these proteins were produced at low levels because of post-induction cell growth inhibition, and the proteins showed poor post-purification solubility. Here, we design and produce a new, type of recombinant MAP, fp-353, that is a fusion protein with fp-3A at each terminus of fp-5. Because fp-353 formed inclusion bodies, host cell growth inhibition did not occur. In addition, the solubility of MAP fp-353 after purification was significantly enhanced, permitting the preparation of a viscous concentrated glue solution for large-scale adhesion strength measurements. Together with large-scale cowhide adhesion measurements and cell-adhesion analyses, we successfully demonstrated that fusion mussel protein fp-353 has potential as a practical alternative bioadhesive.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25701
- DOI
- 10.1021/BP.65
- ISSN
- 8756-7938
- Article Type
- Article
- Citation
- BIOTECHNOLOGY PROGRESS, vol. 24, no. 6, page. 1272 - 1277, 2008-11
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.