DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Kyoung Ro Kim | - |
dc.contributor.author | Seonghye Lim | - |
dc.contributor.author | Yoo Seong Choi | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2016-04-01T02:45:10Z | - |
dc.date.available | 2016-04-01T02:45:10Z | - |
dc.date.created | 2009-06-04 | - |
dc.date.issued | 2009-02-01 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.other | 2009-OAK-0000021793 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25702 | - |
dc.description.abstract | Efficient target gene delivery into eukaryotic cells is important for biotechnological research and gene therapy. Gene delivery based on proteins, including histones, has recently emerged as a powerful non-viral DNA transfer technique. Here, we investigated the potential use of a recombinant mussel adhesive protein, hybrid fp-151, as a gene delivery material, in view of its similar basic amino acid composition to histone proteins, and cost-effective and high-level production in Escherichia coli. After confirming DNA binding affinity, we transfected mammalian cells (human 293T and mouse NIH/3T3) with foreign genes using hybrid fp-151 as the gene delivery carrier. Hybrid fp-151 displayed comparable transfection efficiency in both mammalian cell lines, compared to the widely used transfection agent, Lipofectamine (TM) 2000. Our results indicate that this mussel adhesive protein may be used as a potential protein-based gene-transfer mediator. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.title | RECOMBINANT MUSSEL ADHESIVE PROTEIN AS A GENE DELIVERY MATERIAL | - |
dc.type | Article | - |
dc.contributor.college | 해양대학원 | - |
dc.identifier.doi | 10.1002/BIT.22086 | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Kim, KR | - |
dc.author.google | Lim, S | - |
dc.author.google | Choi, YS | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 102 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 616 | - |
dc.relation.lastpage | 623 | - |
dc.contributor.id | 10167197 | - |
dc.relation.journal | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.102, no.2, pp.616 - 623 | - |
dc.identifier.wosid | 000262540300026 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 623 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 616 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 102 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | Yoo Seong Choi | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-58249088354 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 7 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CHONDROCYTE TRANSPLANTATION SURGERY | - |
dc.subject.keywordPlus | HISTONE H1-MEDIATED TRANSFECTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | DNA-TRANSFECTION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | EFFICIENT | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | H1 | - |
dc.subject.keywordAuthor | mussel adhesive protein | - |
dc.subject.keywordAuthor | hybrid fp-151 | - |
dc.subject.keywordAuthor | DNA binding | - |
dc.subject.keywordAuthor | gene delivery material | - |
dc.subject.keywordAuthor | transfection | - |
dc.subject.keywordAuthor | mammalian cells | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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