DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, YC | - |
dc.contributor.author | Kim, KL | - |
dc.contributor.author | Ha, SH | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Ryu, SH | - |
dc.date.accessioned | 2016-04-01T02:47:30Z | - |
dc.date.available | 2016-04-01T02:47:30Z | - |
dc.date.created | 2011-05-24 | - |
dc.date.issued | 2010-11 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.other | 2010-OAK-0000021598 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25771 | - |
dc.description.abstract | Integrin signaling plays critical roles in cell adhesion, spreading, and migration, and it is generally accepted that to regulate these integrin functions accurately, localized actin remodeling is required. However, the molecular mechanisms that control the targeting of actin regulation molecules to the proper sites are unknown. We previously demonstrated that integrin-mediated cell spreading and migration on fibronectin are dependent on the localized activation of phospholipase D (PLD). However, the mechanism underlying PLD activation by integrin is largely unknown. Here we demonstrate that protein kinase C delta (PKC delta) is required for integrin-mediated PLD signaling. After integrin stimulation, PKC delta is activated and translocated to the edges of lamellipodia, where it colocalizes with PLD2. The abrogation of PKC delta activity inhibited integrin-induced PLD activation and cell spreading. Finally, we show that Thr566 of PLD2 is directly phosphorylated by PKC delta and that PLD2 mutation in this region prevents PLD2 activation, PLD2 translocation to the edge of lamellipodia, Rac translocation, and cell spreading after integrin activation. Together, these results suggest that PKC delta is a primary regulator of integrin-mediated PLD activation via the direct phosphorylation of PLD, which is essential for directing integrin-induced cell spreading. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.relation.isPartOf | Molecular Cell Biology | - |
dc.title | PKCδ-mediated phosphorylation of phospholipase D controls integrin-mediated cell spreading | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1128/MCB.00443-10 | - |
dc.author.google | Chae, YC | - |
dc.author.google | Kim, KL | - |
dc.author.google | Ha, SH | - |
dc.author.google | Kim, J | - |
dc.author.google | Suh, PG | - |
dc.author.google | Ryu, SH | - |
dc.relation.volume | 30 | - |
dc.relation.issue | 21 | - |
dc.relation.startpage | 5086 | - |
dc.relation.lastpage | 5098 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | Molecular Cell Biology | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Molecular Cell Biology, v.30, no.21, pp.5086 - 5098 | - |
dc.identifier.wosid | 000282748600009 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 5098 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 5086 | - |
dc.citation.title | Molecular Cell Biology | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-77957825084 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 17 | - |
dc.description.scptc | 17 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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