DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Shin, GW | - |
dc.contributor.author | Choi, SJ | - |
dc.contributor.author | Hwang, HS | - |
dc.contributor.author | Jung, GY | - |
dc.contributor.author | Seo, TS | - |
dc.date.accessioned | 2016-04-01T02:51:15Z | - |
dc.date.available | 2016-04-01T02:51:15Z | - |
dc.date.created | 2010-06-22 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.other | 2010-OAK-0000021394 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25876 | - |
dc.description.abstract | Rapid and simple analysis for the multiple target pathogens is critical for patient management CE-SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram In this study, we explored the potential of poly(ethyleneoxide)-poly (propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer as a sieving matrix for CE-SSCP analysis on a microdevice To demonstrate the superior resolving power of PEO-PPO-PEO copolymers, 255-bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyt, Pseudomonas aerugmosa, and Neisseria meningitidis, were analyzed in the PEO-PPO-PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan (TM) gel. Due to enhanced dynamic coating and sieving ability, PEO-PPO-PEO copolymer displayed fourfold enhancement of resolving power in the CE-SSCP to separate same-sized DNA molecules Fivefold Input of genomic DNA of P aeruginosa and/or N meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high-resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO-PPO-PEO triblock copolymer an excellent matrix in the CE-SSCP analysis on the microdevice | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.subject | CE-SSCP | - |
dc.subject | Microdevice | - |
dc.subject | Pathogen | - |
dc.subject | Poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) | - |
dc.subject | Quantification | - |
dc.subject | STRAND CONFORMATION POLYMORPHISM | - |
dc.subject | MICROCHIP ELECTROPHORESIS | - |
dc.subject | FOODBORNE PATHOGENS | - |
dc.subject | BLOCK-COPOLYMERS | - |
dc.subject | PCR | - |
dc.subject | SEPARATION | - |
dc.subject | SYSTEM | - |
dc.subject | GENE | - |
dc.subject | SSCP | - |
dc.subject | CE | - |
dc.title | Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1002/ELPS.200900651 | - |
dc.author.google | Kim, SJ | - |
dc.author.google | Shin, GW | - |
dc.author.google | Choi, SJ | - |
dc.author.google | Hwang, HS | - |
dc.author.google | Jung, GY | - |
dc.author.google | Seo, TS | - |
dc.relation.volume | 31 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 1108 | - |
dc.relation.lastpage | 1115 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | ELECTROPHORESIS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.31, no.6, pp.1108 - 1115 | - |
dc.identifier.wosid | 000276807300017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1115 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1108 | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-77949750891 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 12 | - |
dc.description.scptc | 14 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CONFORMATION POLYMORPHISM ANALYSIS | - |
dc.subject.keywordPlus | MICROCHIP ELECTROPHORESIS | - |
dc.subject.keywordPlus | FOODBORNE PATHOGENS | - |
dc.subject.keywordPlus | BLOCK-COPOLYMERS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | POLYACRYLAMIDE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | COMMUNITIES | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordAuthor | CE-SSCP | - |
dc.subject.keywordAuthor | Microdevice | - |
dc.subject.keywordAuthor | Pathogen | - |
dc.subject.keywordAuthor | Poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) | - |
dc.subject.keywordAuthor | Quantification | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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