DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, K | - |
dc.contributor.author | Lee, MY | - |
dc.contributor.author | Kim, KS | - |
dc.contributor.author | Hahn, SK | - |
dc.date.accessioned | 2016-04-01T02:51:25Z | - |
dc.date.available | 2016-04-01T02:51:25Z | - |
dc.date.created | 2010-06-16 | - |
dc.date.issued | 2010-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 2010-OAK-0000021386 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25879 | - |
dc.description.abstract | Target specific delivery of small interfering RNA (siRNA) has been regarded as one of the most important technologies for the development of siRNA therapeutics. In this work, non-toxic low molecular weight (MW) polyethyleneimine (PEI, 2000 Da) was cross-linked with cystamine bisacrylamide (CBA) to prepare reducible PEI-SS in the body. Then, PEI-SS was conjugated with hyaluronic acid (HA) in the form of block-copolymer to enhance serum stability and facilitate target specific cellular uptake of siRNA by HA receptor mediated endocytosis. The cytotoxicity of (PEI-SS)-b-HA conjugate appeared to be negligible likely due to the degradation of PEI-SS to low MW PEI in the cytosol. Flow cytometric and confocal microscopic analyses confirmed the HA receptor mediated endocytosis of siRNA/(PEI-SS)-b-HA complex. The siRNA/(PEI-SS)-b-HA complex demonstrated an excellent in vitro gene silencing efficiency in the range of 50-80% reducing the mRNA expression level in the absence and presence of 50 vol% serum. Moreover, intra-tumoral injection of vascular endothelial growth factor (VEGF) siRNA/(PEI-SS)-b-HA complex resulted in dramatically inhibited tumor growth with reduced VEGF mRNA and VEGF levels in the tumors. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.subject | Hyaluronic acid | - |
dc.subject | Polyethyleneimine | - |
dc.subject | siRNA | - |
dc.subject | Target delivery | - |
dc.subject | Gene silencing | - |
dc.subject | LOW-MOLECULAR-WEIGHT | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | MEDIATED GENE DELIVERY | - |
dc.subject | SMALL INTERFERING RNA | - |
dc.subject | INTRACELLULAR DELIVERY | - |
dc.subject | HIV-1 INFECTION | - |
dc.subject | DNA DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | RECEPTOR | - |
dc.subject | THERAPEUTICS | - |
dc.title | Target Specific Tumor Treatment by VEGF siRNA Complexed with Reducible Polyethyleneimine-Hyaluronic Acid Conjugate | - |
dc.type | Article | - |
dc.contributor.college | 신소재공학과 | - |
dc.identifier.doi | 10.1016/J.BIOMATERIALS.2010.03.018 | - |
dc.author.google | Park, K | - |
dc.author.google | Lee, MY | - |
dc.author.google | Kim, KS | - |
dc.author.google | Hahn, SK | - |
dc.relation.volume | 31 | - |
dc.relation.issue | 19 | - |
dc.relation.startpage | 5258 | - |
dc.relation.lastpage | 5265 | - |
dc.contributor.id | 10149037 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.31, no.19, pp.5258 - 5265 | - |
dc.identifier.wosid | 000278466100024 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 5265 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 5258 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-77952289449 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 96 | - |
dc.description.scptc | 99 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | SMALL INTERFERING RNA | - |
dc.subject.keywordPlus | INTRACELLULAR DELIVERY | - |
dc.subject.keywordPlus | MEDIATED DELIVERY | - |
dc.subject.keywordPlus | HIV-1 INFECTION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | Hyaluronic acid | - |
dc.subject.keywordAuthor | Polyethyleneimine | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Target delivery | - |
dc.subject.keywordAuthor | Gene silencing | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
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