Mucosal prior to systemic application of recombinant adenovirus boosting is more immunogenic than systemic application twice but confers similar protection against SIV-challenge in DNA vaccine-primed
SCIE
SCOPUS
- Title
- Mucosal prior to systemic application of recombinant adenovirus boosting is more immunogenic than systemic application twice but confers similar protection against SIV-challenge in DNA vaccine-primed
- Authors
- Schulte, R; Suh, YS; Sauermann, U; Ochieng, W; Sopper, S; Kim, KS; Ahn, SS; Park, KS; Stolte-Leeb, N; Hunsmann, G; Sung, YC; Stahl-Hennig, C
- Date Issued
- 2009-01-20
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- We investigated the immunogenicity and efficacy of a bimodal prime/boost vaccine regimen given by various routes in the Simian immunodeficiency virus (SIV) rhesus monkey model for AIDS. Twelve animals were immunized with SIV DNA-vectors followed by the application of a recombinant adenovirus (rAd5) expressing the same genes either intramuscularly (i.m.) or by oropharyngeal spray. The second rAd5-application was given i.m. All vaccinees plus six controls were challenged orally with SIVmac239 12 weeks post-final immunization. Both immunization strategies induced strong SIV Gag-specific IFN-gamma and T-cell proliferation responses and mediated a conservation of CD4(+) memory T-cells and a reduction of viral load during peak viremia following infection. Interestingly, the mucosal group was superior to the systemic group regarding breadth and strength of SIV-specific T-cell responses and exhibited lower vector specific immune responses. Therefore, our data warrant the inclusion of mucosal vector application in a vaccination regimen which makes it less invasive and easier to apply. (c) 2008 Elsevier Inc. All rights reserved.
- Keywords
- SIV; Mucosal immunization; Adenovirus; Vaccine; Macaque; SIMIAN-IMMUNODEFICIENCY-VIRUS; T-CELL RESPONSES; REPLICATION-DEFECTIVE ADENOVIRUS; RHESUS MACAQUES; IMMUNE-RESPONSES; NEUTRALIZING ANTIBODIES; CYNOMOLGUS MONKEYS; TIANTAN VACCINIA; VECTOR VACCINE; INFECTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26166
- DOI
- 10.1016/J.VIROL.2008.10.012
- ISSN
- 0042-6822
- Article Type
- Article
- Citation
- VIROLOGY, vol. 383, no. 2, page. 300 - 309, 2009-01-20
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.