Murine B Cell Response to TLR7 Ligands Depends on an IFN-beta Feedback Loop
SCIE
SCOPUS
- Title
- Murine B Cell Response to TLR7 Ligands Depends on an IFN-beta Feedback Loop
- Authors
- Green, NM; Laws, A; Kiefer, K; Busconi, L; Kim, YM; Brinkmann, MM; Trail, EH; Yasuda, K; Christensen, SR; Shlomchik, MJ; Vogel, S; Connor, JH; Ploegh, H; Eilat, D; Rifkin, IR; van Seventer, JM; Marshak-Rothstein, A
- Date Issued
- 2009-08-01
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Abstract
- Type I IFNs play an important, yet poorly characterized, role in systemic lupus erythematosus. To better understand the interplay between type I IFNs and the activation of autoreactive B cells, we evaluated the effect of type I IFN receptor (IFNAR) deficiency in murine B cell responses to common TLR ligands. In comparison to wild-type B cells, TLR7-stimulated IFNAR(-/-) B cells proliferated significantly less well and did not up-regulate costimulatory molecules. By contrast, IFNAR1(-/-) B cells did not produce cytokines, but did proliferate and up-regulate activation markers in response to other TLR ligands. These defects were not due to a difference in the distribution of B cell populations or a failure to produce a soluble factor other than a type I IFN. Instead, the compromised response pattern reflected the disruption of an IFN-beta feedback loop and constitutively low expression of TLR7 in the IFNAR1(-/-) B cells. These results highlight subtle differences in the IFN dependence of TLR7 responses compared with other TLR-mediated B cell responses. The Journal of Immunology, 2009, 183: 1569-1576.
- Keywords
- SYSTEMIC-LUPUS-ERYTHEMATOSUS; I INTERFERON RECEPTOR; TOLL-LIKE RECEPTORS; PLASMACYTOID DENDRITIC CELLS; MEDIATED ENHANCEMENT; IMMUNE-COMPLEXES; ALPHA PRODUCTION; MICE; ACTIVATION; DISEASE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26230
- DOI
- 10.4049/JIMMUNOL.0803899PG 8
- ISSN
- 0022-1767
- Article Type
- Article
- Citation
- JOURNAL OF IMMUNOLOGY, vol. 183, no. 3, page. 1569 - 1576, 2009-08-01
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