DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, JS | - |
dc.contributor.author | Yoo, SA | - |
dc.contributor.author | Kim, JW | - |
dc.contributor.author | Yang, SP | - |
dc.contributor.author | Chae, CB | - |
dc.contributor.author | Tarallo, V | - |
dc.contributor.author | De Falco, S | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Cho, CS | - |
dc.contributor.author | Kim, WU | - |
dc.date.accessioned | 2016-04-01T03:08:30Z | - |
dc.date.available | 2016-04-01T03:08:30Z | - |
dc.date.created | 2010-04-23 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 0004-3591 | - |
dc.identifier.other | 2010-OAK-0000020651 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26306 | - |
dc.description.abstract | Objective. To delineate the role of neuropilin-1 (NP-1), a vascular endothelial growth factor receptor (VEGFR), in rheumatoid inflammation and to determine whether blockade of NP-1 could suppress synoviocyte survival and angiogenesis. Methods. VEGF(111-165) peptide, which encompasses the NP-1 binding domain of VEGF(165), was generated by cleaving VEGF(165) with plasmin. The effect of this peptide on the interaction between VEGF(165) and its receptor was determined by I-125-VEGFR binding assay. Assays to determine synoviocyte apoptosis, adhesion, and migration were performed in the presence of VEGF(165) and/ or the peptide. VEGF(165)-induced angiogenesis was assessed by measuring the proliferation, tube formation, and wounding migration of endothelial cells (ECs). Mice were immunized with type II collagen to induce experimental arthritis. Results. VEGF(111-165) peptide specifically inhibited the binding of I-125-VEGF(165) to NP-1 on rheumatoid synoviocytes and ECs. The peptide eliminated the VEGF(165)-mediated increase in synoviocyte survival and activation of p-ERK and Bcl-2. The peptide also completely inhibited a VEGF(165)-induced increase in synoviocyte adhesion and migration. In addition, the anti-NP-1 peptide blocked VEGF(165)-stimulated proliferation, capillary tube formation, and wounding migration of ECs in vitro. VEGF(165)-induced neovascularization in a Matrigel plug in mice was also blocked by treatment with the peptide. Finally, subcutaneous injection of anti-NP-1 peptide suppressed arthritis severity and autoantibody formation in mice with experimental arthritis and inhibited synoviocyte hyperplasia and angiogenesis in arthritic joints. Conclusion. Anti-NP-1 peptide suppressed VEGF(165)-induced increases in synoviocyte survival and angiogenesis, and thereby blocked experimental arthritis. Our findings suggest that anti-NP-1 peptide could be useful in alleviating chronic arthritis. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | willy interscience | - |
dc.relation.isPartOf | ARTHRITIS AND RHEUMATISM | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | ELEMENT-BINDING PROTEIN | - |
dc.subject | RHEUMATOID-ARTHRITIS | - |
dc.subject | IN-VIVO | - |
dc.subject | SOLUBLE NEUROPILIN-1 | - |
dc.subject | FACTOR DETERMINANTS | - |
dc.subject | BCL-2 EXPRESSION | - |
dc.subject | CELL-MIGRATION | - |
dc.subject | FACTOR VEGF | - |
dc.subject | RECEPTOR | - |
dc.title | Anti-Neuropilin-1 Peptide Inhibition of Synoviocyte Survival, Angiogenesis, and Experimental Arthritis | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1002/art.27243 | - |
dc.author.google | Kong, JS | - |
dc.author.google | Yoo, SA | - |
dc.author.google | Kim, JW | - |
dc.author.google | Yang, SP | - |
dc.author.google | Chae, CB | - |
dc.author.google | Tarallo, V | - |
dc.author.google | De Falco, S | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Cho, CS | - |
dc.author.google | Kim, WU | - |
dc.relation.volume | 62 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 179 | - |
dc.relation.lastpage | 190 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | ARTHRITIS AND RHEUMATISM | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ARTHRITIS AND RHEUMATISM, v.62, no.1, pp.179 - 190 | - |
dc.identifier.wosid | 000279275700020 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 190 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 179 | - |
dc.citation.title | ARTHRITIS AND RHEUMATISM | - |
dc.citation.volume | 62 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-74849095453 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 23 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | ELEMENT-BINDING PROTEIN | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | SOLUBLE NEUROPILIN-1 | - |
dc.subject.keywordPlus | FACTOR DETERMINANTS | - |
dc.subject.keywordPlus | BCL-2 EXPRESSION | - |
dc.subject.keywordPlus | CELL-MIGRATION | - |
dc.subject.keywordPlus | FACTOR VEGF | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.relation.journalWebOfScienceCategory | Rheumatology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Rheumatology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.