AtHMA1 contributes to the detoxification of excess Zn(II) in Arabidopsis
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SCOPUS
- Title
- AtHMA1 contributes to the detoxification of excess Zn(II) in Arabidopsis
- Authors
- Kim, YY; Choi, H; Segami, S; Cho, HT; Martinoia, E; Maeshima, M; Lee, Y
- Date Issued
- 2009-06
- Publisher
- WILEY-BLACKWELL PUBLISHING, INC
- Abstract
- AtHMA1 is a member of the heavy metal-transporting ATPase family. It exhibits amino acid sequence similarity to two other Zn(II) transporters, AtHMA2 and AtHMA4, and contains poly-His motifs that are commonly found in Zn(II)-binding proteins, but lacks some amino acids that are typical for this class of transporters. AtHMA1 localizes to the chloroplast envelope. In comparison with wild-type plants, we observed a more pronounced sensitivity in the presence of high ZOO concentrations, and increased accumulation of Zn in the chloroplast of T-DNA insertional mutants in AtHMA1. The Zn(II)-sensitive phenotype of AtHMA1 knock-out plants was complemented by the expression of AtHMA1 under the control of its own promoter. The Zn(II)-transporting activity of AtHMA1 was confirmed in a heterologous expression system, Saccharomyces cerevisiae. The sensitivity of yeast to high concentrations of Zn(II) was altered by the expression of AtHMA1 lacking its N-terminal chloroplast-targeting signal. Taken together, these results suggest that under conditions of excess Zn(II), AtHMA1 contributes to Zn(II) detoxification by reducing the Zn content of Arabidopsis thaliana plastids.
- Keywords
- HMA1; P-type ATPase; heavy metal tolerance; zinc detoxification; Arabidopsis; transgenic plants; P-TYPE ATPASE; ZINC TRANSPORTERS; METAL TRANSPORTER; ESCHERICHIA-COLI; ABC TRANSPORTER; HEAVY-METALS; THALIANA; YEAST; PLANTS; PHYTOREMEDIATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26365
- DOI
- 10.1111/J.1365-313X.2009.03818.X
- ISSN
- 0960-7412
- Article Type
- Article
- Citation
- PLANT JOURNAL, vol. 58, no. 5, page. 737 - 753, 2009-06
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