DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ae Ryang Jung | - |
dc.contributor.author | Richard Y. Kim | - |
dc.contributor.author | Hyung Woo Kim | - |
dc.contributor.author | Kshitiz Raj Shrestha | - |
dc.contributor.author | Seung Hwan Jeon | - |
dc.contributor.author | Kyoung Je Cha | - |
dc.contributor.author | Yong Hyun Park | - |
dc.contributor.author | Kim, DS | - |
dc.contributor.author | Ji Youl Lee | - |
dc.date.accessioned | 2016-04-01T07:34:38Z | - |
dc.date.available | 2016-04-01T07:34:38Z | - |
dc.date.created | 2015-07-31 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.other | 2015-OAK-0000033491 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26650 | - |
dc.description.abstract | Human adipose-derived stem cells (hADSCs) can differentiate into various cell types depending on chemical and topographical cues. One topographical cue recently noted to be successful in inducing differentiation is the nanoengineered polystyrene surface containing nanopore array-patterned substrate (NP substrate), which is designed to mimic the nanoscale topographical features of the extracellular matrix. In this study, efficacies of NP and flat substrates in inducing neural differentiation of hADSCs were examined by comparing their substrate-cell adhesion rates, filopodia growth, nuclei elongation, and expression of neural-specific markers. The polystyrene nano Petri dishes containing NP substrates were fabricated by a nano injection molding process using a nickel electroformed nano-mold insert (Diameter: 200nm. Depth of pore: 500nm. Center-to-center distance: 500nm). Cytoskeleton and filopodia structures were observed by scanning electron microscopy and F-actin staining, while cell adhesion was tested by vinculin staining after 24 and 48h of seeding. Expression of neural specific markers was examined by real-time quantitative polymerase chain reaction and immunocytochemistry. Results showed that NP substrates lead to greater substrate-cell adhesion, filopodia growth, nuclei elongation, and expression of neural specific markers compared to flat substrates. These results not only show the advantages of NP substrates, but they also suggest that further study into cell-substrate interactions may yield great benefits for biomaterial engineering. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART A | - |
dc.title | Nanoengineered Polystyrene Surfaces with Nanopore Array Pattern Alters Cytoskeleton Organization and Enhances Induction of Neural Differentiation of Human Adipose-Derived Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.1089/TEN.TEA.2014.0346 | - |
dc.author.google | Jung A.R., Kim R.Y., Kim H.W., Shrestha K.R., Jeon S.H., Cha K.J., Park Y.H., Kim D.S., Lee J.Y. | - |
dc.relation.volume | 21 | - |
dc.relation.issue | 13 | - |
dc.relation.startpage | 2115 | - |
dc.relation.lastpage | 2124 | - |
dc.contributor.id | 10170232 | - |
dc.relation.journal | TISSUE ENGINEERING PART A | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, v.21, no.13, pp.2115 - 2124 | - |
dc.identifier.wosid | 000363982900015 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 2124 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 2115 | - |
dc.citation.title | TISSUE ENGINEERING PART A | - |
dc.citation.volume | 21 | - |
dc.contributor.affiliatedAuthor | Kim, DS | - |
dc.identifier.scopusid | 2-s2.0-84936873888 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.description.scptc | 8 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | NANOSCALE | - |
dc.subject.keywordPlus | TOPOGRAPHY | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | FATE | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.