DC Field | Value | Language |
---|---|---|
dc.contributor.author | Muthiah, M | - |
dc.contributor.author | Che, HL | - |
dc.contributor.author | Kalash, S | - |
dc.contributor.author | Jo, J | - |
dc.contributor.author | Choi, SY | - |
dc.contributor.author | Kim, WJ | - |
dc.contributor.author | Cho, CS | - |
dc.contributor.author | Lee, JY | - |
dc.contributor.author | Park, IK | - |
dc.date.accessioned | 2016-04-01T07:47:03Z | - |
dc.date.available | 2016-04-01T07:47:03Z | - |
dc.date.created | 2015-06-22 | - |
dc.date.issued | 2015-02-01 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.other | 2015-OAK-0000033118 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26868 | - |
dc.description.abstract | In this study, thiol-modified siRNA (SH-siRNA) was delivered by bioreducible polyethylenimine (ssPEI), to enhance physicochemical properties of polyplexes and function of siRNA through disulfide bonding between SH-siRNA and ssPEI. The ssPEI was utilized to deliver Akt1 SH-siRNA for suppression of Akt1 mRNA and blockage of Akt1 protein translation, resulting in reduced cellular proliferation and the induction of apoptosis. Disulfide bondings between the ssPEI and SH-siRNA through thiol groups in both were confirmed by DTT treatment. Complexation between ssPEI and Akt1SH-siRNA was enhanced and reduced surface charge of ssPEI/Akt1SH-siRNA complexes with smaller average particle sizes even at lower N/P ratios was obtained compared with PEI/Akt1siRNA ones. Cellular uptake of ssPEI/Akt1SH-siRNA complexes in CT-26 mouse colon cancer cells was also enhanced. The ssPEI/Akt1SH-siRNA complexes reduced proliferation and increased apoptosis of mouse colon cancer cells in vitro. In an in vivo mouse tumor model, the complexes reduced tumor proliferation and downregulation of Akt1 compared to controls. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.title | Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/J.COLSURFB.2014.12.022 | - |
dc.author.google | Muthiah, M | - |
dc.author.google | Che, HL | - |
dc.author.google | Kalash, S | - |
dc.author.google | Jo, J | - |
dc.author.google | Choi, SY | - |
dc.author.google | Kim, WJ | - |
dc.author.google | Cho, CS | - |
dc.author.google | Lee, JY | - |
dc.author.google | Park, IK | - |
dc.relation.volume | 126 | - |
dc.relation.startpage | 322 | - |
dc.relation.lastpage | 327 | - |
dc.contributor.id | 10135304 | - |
dc.relation.journal | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.126, pp.322 - 327 | - |
dc.identifier.wosid | 000350918900041 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 327 | - |
dc.citation.startPage | 322 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 126 | - |
dc.contributor.affiliatedAuthor | Kim, WJ | - |
dc.identifier.scopusid | 2-s2.0-84920662627 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 12 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordAuthor | Bioreducible PEI | - |
dc.subject.keywordAuthor | Thiol modification | - |
dc.subject.keywordAuthor | Akt1 | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | Tumor suppression | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.