DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, J | - |
dc.contributor.author | Kim, SM | - |
dc.contributor.author | Lee, SB | - |
dc.date.accessioned | 2016-04-01T07:50:40Z | - |
dc.date.available | 2016-04-01T07:50:40Z | - |
dc.date.created | 2015-06-18 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1431-0651 | - |
dc.identifier.other | 2015-OAK-0000032893 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26934 | - |
dc.description.abstract | We identified the non-phosphorylated l-rhamnose metabolic pathway (Rha_NMP) genes that are homologous to those in the thermoacidophilic archaeon Thermoplasma acidophilum in the genome of the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans. However, unlike previously known 2-keto-3-deoxy-l-rhamnonate (l-KDR) dehydrogenase (KDRDH) which belongs to the short chain dehydrogenase/reductase superfamily, the putative KDRDHs in S. thermosulfidooxidans (Sulth_3557) and T. acidophilum (Ta0749) belong to the medium chain dehydrogenase/reductase (MDR) superfamily. We demonstrated that Sulth_3559 and Sulth_3557 proteins from S. thermosulfidooxidans function as l-rhamnose dehydrogenase and KDRDH, respectively. Sulth_3557 protein is an NAD(+)-specific KDRDH with optimal temperature and pH of 50 A degrees C and 9.5, respectively. The K (m) and V (max) values for l-KDR were 2.0 mM and 12.8 U/mg, respectively. Sulth_3557 also showed weak 2,3-butanediol dehydrogenase activity. Phylogenetic analysis suggests that Sulth_3557 and its homologs form a new subfamily in the MDR superfamily. The results shown in this study imply that thermoacidophilic archaea metabolize l-rhamnose to pyruvate and l-lactate by using the MDR-family KDRDH similarly to that of the thermoacidophilic bacterium S. thermosulfidooxidans. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER JAPAN KK | - |
dc.relation.isPartOf | EXTREMOPHILES | - |
dc.title | Identification and characterization of 2-keto-3-deoxy-l-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to lrhamnose metabolism in archaea | - |
dc.type | Article | - |
dc.contributor.college | 엔지니어링 대학원 | - |
dc.identifier.doi | 10.1007/S00792-015-0731-8 | - |
dc.author.google | Bae, J | - |
dc.author.google | Kim, SM | - |
dc.author.google | Lee, SB | - |
dc.relation.volume | 19 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 469 | - |
dc.relation.lastpage | 478 | - |
dc.contributor.id | 10105619 | - |
dc.relation.journal | EXTREMOPHILES | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EXTREMOPHILES, v.19, no.2, pp.469 - 478 | - |
dc.identifier.wosid | 000350306200022 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 478 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 469 | - |
dc.citation.title | EXTREMOPHILES | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | Lee, SB | - |
dc.identifier.scopusid | 2-s2.0-84925489640 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.description.scptc | 4 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | (2R,3R)-2,3-BUTANEDIOL DEHYDROGENASE | - |
dc.subject.keywordPlus | THERMOPLASMA-ACIDOPHILUM | - |
dc.subject.keywordPlus | ENZYMATIC-ACTIVITIES | - |
dc.subject.keywordPlus | ENOLASE SUPERFAMILY | - |
dc.subject.keywordPlus | GEN. NOV. | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DEHYDRATASE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | 2-Keto-3-deoxy-L-rhamnonate dehydrogenase | - |
dc.subject.keywordAuthor | Non-phosphorylated L-rhamnose pathway | - |
dc.subject.keywordAuthor | Medium chain dehydrogenase/reductase | - |
dc.subject.keywordAuthor | Sulfobacillus thermosulfidooxidans | - |
dc.subject.keywordAuthor | Thermoplasma acidophilum | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.