Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection
SCIE
SCOPUS
- Title
- Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection
- Authors
- Goeun Lee; Na, YJ; Yang, BG; Choi, JP; Seo, YB; Hong, CP; Yun, CH; Kim, DH; Sohn, EJ; Kim, JH; Sung, YC; Kim, YK; Jang, MH; Hwang, I
- Date Issued
- 2015-01
- Publisher
- Blackwell Publishing Inc.
- Abstract
- Pandemics in poultry caused by the highly pathogenic avian influenza (HPAI) A virus occur too frequently globally, and there is growing concern about the HPAI A virus due to the possibility of a pandemic among humans. Thus, it is important to develop a vaccine against HPAI suitable for both humans and animals. Various approaches are underway to develop such vaccines. In particular, an edible vaccine would be a convenient way to vaccinate poultry because of the behaviour of the animals. However, an edible vaccine is still not available. In this study, we developed a strategy of effective vaccination of mice by the oral administration of transgenic Arabidopsis plants (HA-TG) expressing haemagglutinin (HA) in the endoplasmic reticulum (ER). Expression of HA in the ER resulted in its high-level accumulation, N-glycosylation, protection from proteolytic degradation and long-term stability. Oral administration of HA-TG with saponin elicited high levels of HA-specific systemic IgG and mucosal IgA responses in mice, which resulted in protection against a lethal influenza virus infection with attenuated inflammatory symptoms. Based on these results, we propose that oral administration of freeze-dried leaf powders from transgenic plants expressing HA in the ER together with saponin is an attractive strategy for vaccination against influenza A virus.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/27236
- DOI
- 10.1111/PBI.12235
- ISSN
- 1467-7644
- Article Type
- Article
- Citation
- Plant Biotechnology Journal, vol. 13, no. 1, page. 62 - 72, 2015-01
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