Protein Expression Profiling of Primary Mammary Epithelial Cells Derived from MMTV-neu Mice Revealed that HER2/NEU-Driven Changes in Protein Expression Are Functionally Clustered
- Title
- Protein Expression Profiling of Primary Mammary Epithelial Cells Derived from MMTV-neu Mice Revealed that HER2/NEU-Driven Changes in Protein Expression Are Functionally Clustered
- Authors
- Park, S; Lee, KM; Ju, JH; Kim, J; Noh, DY; Lee, T; Shin, I; null
- Date Issued
- 2010-01
- Publisher
- JOHN WILEY & SONS INC
- Abstract
- MMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest (TM)/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis. (C) 2009 IUBMB IUBMB Life, 62(1): 41-50, 2010
- Keywords
- proteonomics; signal transduction; HER2/NEU; HUMAN-BREAST-CANCER; NF-KAPPA-B; RNA-POLYMERASE-II; TRANSGENIC MICE; ONCOGENE; OVEREXPRESSION; GROWTH; GENE; MECHANISM; APOPTOSIS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/27516
- DOI
- 10.1002/IUB.276
- ISSN
- 1521-6543
- Article Type
- Article
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.