DC Field | Value | Language |
---|---|---|
dc.contributor.author | Buehler, C | - |
dc.contributor.author | Kim, KH | - |
dc.contributor.author | Greuter, U | - |
dc.contributor.author | Schlumpf, N | - |
dc.contributor.author | So, PTC | - |
dc.date.accessioned | 2016-04-01T08:28:15Z | - |
dc.date.available | 2016-04-01T08:28:15Z | - |
dc.date.created | 2009-09-24 | - |
dc.date.issued | 2005-01 | - |
dc.identifier.issn | 1053-0509 | - |
dc.identifier.other | 2005-OAK-0000018959 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28047 | - |
dc.description.abstract | We present a multicolor multiphoton fluorescence microscope with single-photon counting sensitivity. The system integrates a standard multiphoton fluorescence microscope, an optical grating spectrograph operating in the UV-Vis wavelength region, and a 16-anode photomultiplier tube (PMT). The major technical innovation is in the development of a multichannel photon counting card (mC-PhCC) for direct signal collection from multi-anode PMTs. The electronic design of the mC-PhCC employs a high-throughput, fully-parallel, single-photon counting scheme along with a high-speed electrical or fiber-optical link interface to the data acquisition computer. There is no electronic crosstalk among the detection channels of the mC-PhCC. The collected signal remains linear up to an incident photon rate of 10(8) counts per second. The high-speed data interface offers ample bandwidth for real-time readout: 2 MByte lambda-stacks composed of 16 spectral channels, 256 x 256 pixel image with 12-bit dynamic range can be transferred at 30 frames per second. The modular design of the mC-PhCC can be readily extended to accommodate PMTs of more anodes. Data acquisition from a 64-anode PMT has been verified. As a demonstration of system performance, spectrally resolved images of fluorescent latex spheres and ex-vivo human skin are reported. The multicolor multiphoton microscope is suitable for highly sensitive, real-time, spectrally-resolved three-dimensional imaging in biomedical applications. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF FLUORESCENCE | - |
dc.subject | multiphoton microscopy | - |
dc.subject | single-photon counting | - |
dc.subject | imaging spectroscopy | - |
dc.subject | LASER-SCANNING MICROSCOPY | - |
dc.subject | PANCREATIC-ISLETS | - |
dc.subject | TUNABLE FILTER | - |
dc.subject | TISSUE | - |
dc.subject | EXCITATION | - |
dc.subject | PHOTOMULTIPLIER | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | RESOLUTION | - |
dc.title | Single-photon counting multicolor multiphoton fluorescence microscope | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.1007/S10895-005-0212-Z | - |
dc.author.google | Buehler, C | - |
dc.author.google | Kim, KH | - |
dc.author.google | Greuter, U | - |
dc.author.google | Schlumpf, N | - |
dc.author.google | So, PTC | - |
dc.relation.volume | 15 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 41 | - |
dc.relation.lastpage | 51 | - |
dc.contributor.id | 10183385 | - |
dc.relation.journal | JOURNAL OF FLUORESCENCE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF FLUORESCENCE, v.15, no.1, pp.41 - 51 | - |
dc.identifier.wosid | 000227216700007 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 51 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 41 | - |
dc.citation.title | JOURNAL OF FLUORESCENCE | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Kim, KH | - |
dc.identifier.scopusid | 2-s2.0-17444399731 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 39 | - |
dc.description.scptc | 42 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LASER-SCANNING MICROSCOPY | - |
dc.subject.keywordPlus | PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | TUNABLE FILTER | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | EXCITATION | - |
dc.subject.keywordPlus | PHOTOMULTIPLIER | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordAuthor | multiphoton microscopy | - |
dc.subject.keywordAuthor | single-photon counting | - |
dc.subject.keywordAuthor | imaging spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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