EXPRESSION OF FUNCTIONAL HUMAN-CYTOSOLIC CU/ZN SUPEROXIDE DISMUTASE IN TRANSGENIC TOBACCO
SCIE
SCOPUS
- Title
- EXPRESSION OF FUNCTIONAL HUMAN-CYTOSOLIC CU/ZN SUPEROXIDE DISMUTASE IN TRANSGENIC TOBACCO
- Authors
- Park, DH; Yoon, SYH; Nam, HG; Park, JM
- Date Issued
- 2002-05
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- Nicotiana tabacum was transformed with cDNA encoding the human cytosolic copper/zinc superoxide dismutase (human SOD) under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The cDNA sequence was present in a range of copies, from single to several copies, in the primary transformant plants and a transcript of the expected length was produced. The expression of human SOD protein in the transgenic plants was shown by in situ gel staining assay. Ten plants produced human SOD up to 40 ng protein per mg of fresh leaf tissue.
- Keywords
- heterologous expression system; superoxide dismutase; transformation; transgenic tobacco; SENSITIVE METHOD; PLANTS; GENE; LYSOZYME
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28467
- DOI
- 10.1023/A:1015273714571
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 24, no. 9, page. 681 - 686, 2002-05
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- There are no files associated with this item.
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