Influence of nanobubbles on molecular binding on gold surfaces
SCIE
SCOPUS
- Title
- Influence of nanobubbles on molecular binding on gold surfaces
- Authors
- Seo, H; Jung, N; Lee, D; Jeon, S
- Date Issued
- 2009-03-20
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- The influence of nanobubbles on molecular binding on gold-coated surfaces has been studied using a quartz crystal microbalance. We found that changes in resonant frequency during oligonucleotide immobilization in degassed buffers were similar to those in air-rich solutions. However, during hybridization of immobilized and complementary oligonucleotide strands, no decrease in frequency was observed in degassed buffer solutions, indicating that no hybridization occurred. In contrast, a substantial decrease was observed in air-rich solutions. The differential effect of nanobubbles on immobilization and hybridization events was attributed to the nature of the underlying molecular interactions: covalent bonding for immobilization and noncovalent bonding for hybridization. Similarly, upon exposure of an antithrombin-immobilized surface to a thrombin-containing solution, decreases in frequency corresponding to specific binding were observed only in air-rich buffer solutions, supporting the hypothesis that nanobubbles play a key role in molecular binding on gold surfaces. (C) 2008 Elsevier B.V. All rights reserved.
- Keywords
- Nanobubbles; DNA immobilization and hybridization; Thrombin-antithrombin; Quartz crystal microbalance; HYDROPHOBIC SURFACES; WATER; HYBRIDIZATION; ATTRACTION; INTERFACE; CAVITIES; BUBBLES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28679
- DOI
- 10.1016/j.colsurfa.2008.11.030
- ISSN
- 0927-7757
- Article Type
- Article
- Citation
- COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, vol. 336, no. 39816, page. 99 - 103, 2009-03-20
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