DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YK | - |
dc.contributor.author | Kim, KR | - |
dc.contributor.author | Kang, DG | - |
dc.contributor.author | Jang, SY | - |
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2016-04-01T08:48:59Z | - |
dc.date.available | 2016-04-01T08:48:59Z | - |
dc.date.created | 2009-06-04 | - |
dc.date.issued | 2009-03 | - |
dc.identifier.issn | 0959-6658 | - |
dc.identifier.other | 2009-OAK-0000016806 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28830 | - |
dc.description.abstract | Most insect cells have a simple N-glycosylation process and consequently paucimannosidic or simple core glycans predominate. Previously, we have shown that paucimannosidic N-glycan structures are dominant in Drosophila S2 cells. It has been proposed that beta-N-acetylglucosaminidase (GlcNAcase), a hexosaminidase in the Golgi membrane which removes a terminal N-acetylglucosamine (GlcNAc), might contribute to simple N-glycosylation in several insects and insect-derived cells except S2 cells. In the present work, we investigated the substantial effects of GlcNAcase on N-glycan patterns in Drosophila S2 cells using two GlcNAcase suppression strategies: an mRNA-targeting approach using RNA interference (RNAi) and a protein-targeting approach using the specific chemical inhibitor 2-acetamido-1,2-dideoxynojirimycin (2-ADN). Using high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses, we found that the N-glycosylation patterns of human erythropoietin (hEPO) secreted by stably transfected S2 cells were more complex following GlcNAcase suppression, which generated N-glycan structures with a terminal GlcNAc and/or galactose. These data demonstrate that GlcNAcase may be an important factor in the formation of paucimannosidic core N-glycans in Drosophila S2 cells and suggest that it may be possible to express complex glycoproteins in engineered Drosophila S2 cells by suppressing GlcNAcase in the N-glycosylation pathway. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.relation.isPartOf | GLYCOBIOLOGY | - |
dc.subject | SECRETED HUMAN ERYTHROPOIETIN | - |
dc.subject | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject | STATISTICAL OPTIMIZATION | - |
dc.subject | INSECT CELLS | - |
dc.subject | PURIFICATION | - |
dc.subject | GLYCANS | - |
dc.subject | EXPRESSION | - |
dc.subject | MEMBRANE | - |
dc.subject | PROTEINS | - |
dc.subject | ENCODES | - |
dc.title | SUPPRESSION OF BETA-N-ACETYLGLUCOSAMINIDASE IN THE N-GLYCOSYLATION PATHWAY FOR COMPLEX GLYCOPROTEIN FORMATION IN DROSOPHILA S2 CELLS | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1093/glycob/cwn138 | - |
dc.relation.volume | 19 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 301 | - |
dc.relation.lastpage | 308 | - |
dc.relation.journal | GLYCOBIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | GLYCOBIOLOGY, v.19, no.3, pp.301 - 308 | - |
dc.identifier.wosid | 000262719700011 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 308 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 301 | - |
dc.citation.title | GLYCOBIOLOGY | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | Kim, KR | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-58949100354 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 28 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SECRETED HUMAN ERYTHROPOIETIN | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | STATISTICAL OPTIMIZATION | - |
dc.subject.keywordPlus | INSECT CELLS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | GLYCANS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ENCODES | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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