DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dennison, SR | - |
dc.contributor.author | Kim, YS | - |
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | Phoenix, DA | - |
dc.date.accessioned | 2016-04-01T09:04:00Z | - |
dc.date.available | 2016-04-01T09:04:00Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 0175-7571 | - |
dc.identifier.other | 2008-OAK-0000010950 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29381 | - |
dc.description.abstract | Halocidin was isolated from hemocytes, Halocynthia aurantium as a heterodimeric peptide consisting of two alpha-helical subunits, Hal15 and Hal18. Hal18 was shown to have antibacterial properties against Bacillus subtilis (MLC = 15 mu M) and Escherichia coli (MLC = 100 mu M). The peptide was shown to produce stable monolayers, which were characteristic of alpha-helical peptides predicted to orientate parallel to the surface of the interface. Constant area assays showed that Hal18 was surface active (4 mu M) inducing surface pressure changes > 30 mN m(-1) characteristic of membrane interactive peptides. The peptide induced stable surface pressure changes in monolayers that were mimetic of B. subtilis membranes (circa 7 mN m(-1)) and E. coli membrane-mimics (circa 4 mN m(-1)). Hal18 inserted readily into zwitterionic DOPE and anionic DOPG monolayers inducing surface pressure changes circa 8 mN m(-1) in both cases, providing evidence that interaction is not headgroup specific. Thermodynamic analysis of compression isotherms showed that the presence of Hal18 destabilised B. subtilis membranes (Delta G (Mix) > 0), which is in contrast to its stabilising effect on E. coli lipid extract implying the differential antimicrobial efficacy may be driven by lipid packing. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | - |
dc.subject | Antimicrobial peptide | - |
dc.subject | Monolayer stability | - |
dc.subject | Peptide monolayer | - |
dc.subject | Lipid-peptide interactions | - |
dc.subject | Thermodynamic analysis | - |
dc.subject | ORIENTATED ALPHA-HELICES | - |
dc.subject | ANTIMICROBIAL PEPTIDES | - |
dc.subject | LIPID MONOLAYERS | - |
dc.subject | TILTED PEPTIDES | - |
dc.subject | DESTABILIZATION | - |
dc.subject | PURIFICATION | - |
dc.subject | PACKING | - |
dc.subject | BINDING | - |
dc.title | INVESTIGATIONS INTO THE ABILITY OF THE PEPTIDE, HAL18, TO INTERACT WITH BACTERIAL MEMBRANES | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1007/s00249-008-0352-6 | - |
dc.author.google | Dennison, SR | - |
dc.author.google | Kim, YS | - |
dc.author.google | Cha, HJ | - |
dc.author.google | Phoenix, DA | - |
dc.relation.volume | 38 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 37 | - |
dc.relation.lastpage | 43 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, v.38, no.1, pp.37 - 43 | - |
dc.identifier.wosid | 000260525000004 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 43 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 37 | - |
dc.citation.title | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | - |
dc.citation.volume | 38 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-55349119857 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ORIENTATED ALPHA-HELICES | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDES | - |
dc.subject.keywordPlus | LIPID MONOLAYERS | - |
dc.subject.keywordPlus | TILTED PEPTIDES | - |
dc.subject.keywordPlus | DESTABILIZATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PACKING | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | Antimicrobial peptide | - |
dc.subject.keywordAuthor | Monolayer stability | - |
dc.subject.keywordAuthor | Peptide monolayer | - |
dc.subject.keywordAuthor | Lipid-peptide interactions | - |
dc.subject.keywordAuthor | Thermodynamic analysis | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
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