DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, S | - |
dc.contributor.author | Lee, SB | - |
dc.date.accessioned | 2016-04-01T09:04:23Z | - |
dc.date.available | 2016-04-01T09:04:23Z | - |
dc.date.created | 2009-03-20 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.other | 2008-OAK-0000010930 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29396 | - |
dc.description.abstract | Expression of archaeal proteins in soluble form is of importance because archaeal proteins are usually produced as insoluble inclusion bodies in Escherichia coli. In this study, we investigated the use of soluble fusion tags to enhance the solubility of two archaeal proteins, D-gluconate dehydratase (GNAD) and 2-keto-3-deoxy-D-gluconate kinase (KDGK), key enzymes in the glycolytic pathway of the thermoacidophilic archaeon. Sulfolobus solfataricus. These two proteins were produced as inclusion bodies in E. coli when polyhistidine was used as a fusion tag. To reduce inclusion body formation in E. coli, GNAD and KDGK were fused with three partners, thioredoxin (Trx), glutathione-S-transferase (GST), and N-utilization substance A (NusA). With the use of fusion-partners, the solubility of the archaeal proteins was remarkably enhanced, and the soluble fraction of the recombinant proteins was increased in this order: Trx > GST > NusA. Furthermore, In the case of recombinant KDGKs, the enzyme activity of the Trx-fused proteins was 200-fold higher than that of the polyhistidine-fusion protein. The strategy presented in this work may contribute to the production of other valuable proteins from hyperthermophilic archaea in E. coli. (C) 2008 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.subject | Archaeal proteins | - |
dc.subject | Soluble fusion tag | - |
dc.subject | D-Gluconate dehydratase | - |
dc.subject | 2-Keto-3-deoxy-D-gluconate kinase | - |
dc.subject | Sulfolobus solfataricus | - |
dc.subject | ENTNER-DOUDOROFF PATHWAY | - |
dc.subject | INCLUSION-BODY FORMATION | - |
dc.subject | HIGH-LEVEL EXPRESSION | - |
dc.subject | SULFOLOBUS-SOLFATARICUS | - |
dc.subject | HETEROLOGOUS EXPRESSION | - |
dc.subject | ARABAD PROMOTER | - |
dc.subject | STRATEGIES | - |
dc.subject | PROTEOMICS | - |
dc.subject | XYLANASE | - |
dc.subject | ENZYMES | - |
dc.title | Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners | - |
dc.type | Article | - |
dc.contributor.college | 경북씨그랜트센터 | - |
dc.identifier.doi | 10.1016/J.PEP.2008.0 | - |
dc.author.google | Kim, S | - |
dc.author.google | Lee, SB | - |
dc.relation.volume | 62 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 116 | - |
dc.relation.lastpage | 119 | - |
dc.contributor.id | 10105619 | - |
dc.relation.journal | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.62, no.1, pp.116 - 119 | - |
dc.identifier.wosid | 000260061900017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 119 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 116 | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 62 | - |
dc.contributor.affiliatedAuthor | Lee, SB | - |
dc.identifier.scopusid | 2-s2.0-52949100181 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 17 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENTNER-DOUDOROFF PATHWAY | - |
dc.subject.keywordPlus | INCLUSION-BODY FORMATION | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | HETEROLOGOUS EXPRESSION | - |
dc.subject.keywordPlus | ARABAD PROMOTER | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | XYLANASE | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | Archaeal proteins | - |
dc.subject.keywordAuthor | Soluble fusion tag | - |
dc.subject.keywordAuthor | D-Gluconate dehydratase | - |
dc.subject.keywordAuthor | 2-Keto-3-deoxy-D-gluconate kinase | - |
dc.subject.keywordAuthor | Sulfolobus solfataricus | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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