DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, S | - |
dc.contributor.author | Lee, SB | - |
dc.date.accessioned | 2016-04-01T09:04:28Z | - |
dc.date.available | 2016-04-01T09:04:28Z | - |
dc.date.created | 2009-03-20 | - |
dc.date.issued | 2008-07 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.other | 2008-OAK-0000010927 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29399 | - |
dc.description.abstract | Achromobacter xylosoxidans is known to utilize D-glucose via the modified Entner-Doudoroff pathway. Although D-gluconate dehydratase produced from this bacterium was purified and partially characterized previously, a gene that encodes this enzyme has not yet been identified. To obtain protein information on bacterial D-gluconate dehydratase, we partially purified D-gluconate dehydratase in A. xylosoxidans and investigated its biochemical properties. Two degenerate primers were designed based on the N-terminal amino acid sequence of the partially purified D-gluconate dehydratase. Through PCR performed using degenerate primers, a 1,782-bp DNA sequence encoding the A. xylosoxidans D-gluconate dehydratase (gnaD) was obtained. The deduced amino acid sequence of A. xylosoxidans gnaD showed strong similarity with that of proteins belonging to the dihydroxy-acid dehydratase/phosphogluconate dehydratase family (COG0129). This is in contrast to the archaeal D-gluconate dehydratase, which belongs to the enolase superfamily (COG4948). The phylogenetic tree showed that A. xylosoxidans D-gluconate dehydratase is closer to the 6-phosphogluconate dehydratase than the dihydroxy-acid dehydratase. Interestingly, a clade containing A. xylosoxidans enzyme was clustered with proteins annotated as a second and a third dihydroxy-acid dehydratase in the genomes of Clostridium acetobutylicum (Cac_ilvD2) and Streptomyces ceolicolor (Sco_ilvD2, Sco_ilvD3), indicating that the function of these enzymes is the dehydration of D-gluconate. (c) KSBB. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEE | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.title | Identification and characterization of the bacterial D-gluconate dehydratase in Achromobacter xylosoxidans | - |
dc.type | Article | - |
dc.contributor.college | 경북씨그랜트센터 | - |
dc.identifier.doi | 10.1007/S12257-008-0 | - |
dc.author.google | Kim, S | - |
dc.author.google | Lee, SB | - |
dc.relation.volume | 13 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 436 | - |
dc.relation.lastpage | 444 | - |
dc.contributor.id | 10105619 | - |
dc.relation.journal | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCOPUS | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.13, no.4, pp.436 - 444 | - |
dc.identifier.wosid | 000258914500009 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 444 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 436 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 13 | - |
dc.contributor.affiliatedAuthor | Lee, SB | - |
dc.identifier.scopusid | 2-s2.0-52749095777 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 9 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENTNER-DOUDOROFF PATHWAY | - |
dc.subject.keywordPlus | THERMOPLASMA-ACIDOPHILUM | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | 2-KETO-3-DEOXY-D-GLUCONATE KINASE | - |
dc.subject.keywordPlus | CLOSTRIDIUM-PASTEURIANUM | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordAuthor | D-gluconate dehydratase | - |
dc.subject.keywordAuthor | Achromobacter xylosoxidans | - |
dc.subject.keywordAuthor | modified Entner-Doudoroff pathway | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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