DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | Dalal, NN | - |
dc.contributor.author | Bentley, WE | - |
dc.date.accessioned | 2016-04-01T09:11:17Z | - |
dc.date.available | 2016-04-01T09:11:17Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2005-07 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.other | 2005-OAK-0000010651 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29614 | - |
dc.description.abstract | Methylotrophic yeast Pichia pastoris is convenient for the expression of eukaryotic foreign proteins owing to its potential for posttranslational modifications, protein folding, and facile culturing. In this work, human interleukin (hIL)-2 was successfully produced as a secreted fusion form in recombinant P. pastoris. By employing green fluorescent protein (GFP) as a monitoring fusion partner, clear identification of fusion protein expression and quantification of intracellular hIL-2 were possible even though there was no correlation between culture supernatant fluorescence and secreted hIL-2 owing to high media interference. Importantly, by the addition of casamino acids in basal medium, we were able to enhance threefold amount of secreted hIL-2, which was present both as a fusion and as a clipped fragment. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | HUMANA PRESS INC | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.subject | human interleukin-2 | - |
dc.subject | green fluorescent protein | - |
dc.subject | Pichia pastoris | - |
dc.subject | fusion protein | - |
dc.subject | secretion | - |
dc.subject | HETEROLOGOUS PROTEINS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | FUSION PARTNER | - |
dc.subject | METHYLOTROPHIC YEAST | - |
dc.subject | EXPRESSION | - |
dc.subject | PURIFICATION | - |
dc.subject | CELLS | - |
dc.subject | CONSTRUCTION | - |
dc.subject | GENE | - |
dc.title | Secretion of human interleukin-2 fused with green fluorescent protein in recombinant Pichia pastoris | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1007/s12010-005-0001-9 | - |
dc.author.google | Cha, HJ | - |
dc.author.google | Dalal, NN | - |
dc.author.google | Bentley, WE | - |
dc.relation.volume | 126 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 1 | - |
dc.relation.lastpage | 11 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.126, no.1, pp.1 - 11 | - |
dc.identifier.wosid | 000230687500001 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 11 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 126 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-22344441302 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 5 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HETEROLOGOUS PROTEINS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FUSION PARTNER | - |
dc.subject.keywordPlus | METHYLOTROPHIC YEAST | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | human interleukin-2 | - |
dc.subject.keywordAuthor | green fluorescent protein | - |
dc.subject.keywordAuthor | Pichia pastoris | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.subject.keywordAuthor | secretion | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.