In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris
SCIE
SCOPUS
- Title
- In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris
- Authors
- Cha, HJ; Dalal, NG; Bentley, WE
- Date Issued
- 2004-07
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
- Keywords
- fusion protein; green fluorescent protein; human interleukin-2; in vivo monitoring; Pichia pastoris; ESCHERICHIA-COLI; GENE-EXPRESSION; PURIFICATION; YEAST; CELLS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29635
- DOI
- 10.1023/B:BILE.0000035489.09417.d4
- ISSN
- 0141-5492
- Article Type
- Article
- Citation
- BIOTECHNOLOGY LETTERS, vol. 26, no. 14, page. 1157 - 1162, 2004-07
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- There are no files associated with this item.
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