Comparative production of green fluorescent protein under co-expression of bacterial hemoglobin in Escherichia coli W3110 using different culture scales
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- Title
- Comparative production of green fluorescent protein under co-expression of bacterial hemoglobin in Escherichia coli W3110 using different culture scales
- Authors
- Johnvesly, B; Kang, DG; Choi, SS; Kim, JH; Cha, HJ
- Date Issued
- 2004-07
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEE
- Abstract
- Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.
- Keywords
- culture scale; Escherichia coli W3110; green fluorescent protein; Vitreoscilla hemoglobin; NUCLEOTIDE-SEQUENCE; ALPHA-AMYLASE; EXPRESSION; PROMOTER; FUSION; GENE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29698
- DOI
- 10.1007/BF02942343
- ISSN
- 1226-8372
- Article Type
- Article
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, vol. 9, no. 4, page. 274 - 277, 2004-07
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