DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Yoo Hyo Jin | - |
dc.contributor.author | Jong Hyub Jun | - |
dc.contributor.author | MOON, WON KYU | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2016-04-01T09:15:27Z | - |
dc.date.available | 2016-04-01T09:15:27Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2004-06 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.other | 2004-OAK-0000010484 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29744 | - |
dc.description.abstract | Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the first time. Using this cDNA, we produced a recombinant Mgfp-5 fused with a hexahistidine affinity ligand, which was expressed in a soluble form in Escherichia coli and was highly purified using affinity chromatography. The adhesive properties of purified recombinant Mgfp-5 were compared with the commercial extracted mussel adhesive Cell-Tak by investigating adhesion force using atomic force microscopy, material surface coating, and quartz crystal microbalance. Even though further macroscale assays are needed, these microscale assays showed that recombinant Mgfp-5 has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isPartOf | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.title | Expression of Functional Recombinant Mussel Adhesive Protein Mgfp-5 in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1128/AEM.70.6.3352-3359.2004 | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Yoo, HJ | - |
dc.author.google | Jun, JH | - |
dc.author.google | Moon, WK | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 70 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 3352 | - |
dc.relation.lastpage | 3359 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.70, no.6, pp.3352 - 3359 | - |
dc.identifier.wosid | 000221981100021 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 3359 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 3352 | - |
dc.citation.title | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.citation.volume | 70 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | MOON, WON KYU | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-2942532196 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 91 | - |
dc.description.scptc | 96 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ATOMIC-FORCE MICROSCOPE | - |
dc.subject.keywordPlus | MYTILUS-EDULIS-L | - |
dc.subject.keywordPlus | BYSSUS | - |
dc.subject.keywordPlus | THICKNESS | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | FILM | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
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