DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, L | - |
dc.contributor.author | Kang, DG | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2016-04-01T09:16:00Z | - |
dc.date.available | 2016-04-01T09:16:00Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2004-01-20 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.other | 2004-OAK-0000010458 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29761 | - |
dc.description.abstract | We investigated the ability of the N-terminal domain of InaK, an ice nucleation protein from Pseudomonas syringae KCTC 1832, to act as an anchoring motif for the display of foreign proteins on the Escherichia coli cell surface. Total expression level and surface display efficiency of green fluorescent protein (GFP) was compared following their fusion with either the N-terminal domain of InaK (InaKN), or with the known truncated InaK containing both N- and C-terminal domains (InaK-NC). We report that the InaK-N/ GFP fusion protein showed a similar cell surface display efficiency (similar to 50%) as InaK-NC/GFP, demonstrating that the InaK N-terminal region alone can direct translocation of foreign proteins to the cell surface and can be employed as a potential cell surface display motif. Moreover, InaK-N/GFP showed the highest levels of total expression and surface display based on unit cell density. InaK-N was also successful in directing cell surface display of organophosphorus hydrolase (OPH), confirming its ability to act as a display motif. (C) 2004 Wiley Periodicals, Inc. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.subject | cell surface display | - |
dc.subject | ice nucleation protein | - |
dc.subject | N-terminal domain | - |
dc.subject | green fluorescent protein | - |
dc.subject | organophosphorus hydrolase | - |
dc.subject | Escherichia coli | - |
dc.subject | GREEN-FLUORESCENT PROTEIN | - |
dc.subject | ORGANOPHOSPHORUS HYDROLASE | - |
dc.subject | PSEUDOMONAS-SYRINGAE | - |
dc.subject | CELL-SURFACE | - |
dc.subject | BACTERIAL HEMOGLOBIN | - |
dc.subject | EXPRESSION | - |
dc.subject | GENE | - |
dc.subject | PESTICIDES | - |
dc.subject | HYDROLYSIS | - |
dc.subject | ENZYME | - |
dc.title | Functional display of foreign protein on surface of Escherichia coli using N-terminal domain of ice nucleation protein | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1002/BIT.10892 | - |
dc.author.google | Li, L | - |
dc.author.google | Kang, DG | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 85 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 214 | - |
dc.relation.lastpage | 221 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.85, no.2, pp.214 - 221 | - |
dc.identifier.wosid | 000187634700012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 221 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 85 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-0942297855 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 71 | - |
dc.description.scptc | 76 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GREEN-FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | ORGANOPHOSPHORUS HYDROLASE | - |
dc.subject.keywordPlus | PSEUDOMONAS-SYRINGAE | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | BACTERIAL HEMOGLOBIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PESTICIDES | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordAuthor | cell surface display | - |
dc.subject.keywordAuthor | ice nucleation protein | - |
dc.subject.keywordAuthor | N-terminal domain | - |
dc.subject.keywordAuthor | green fluorescent protein | - |
dc.subject.keywordAuthor | organophosphorus hydrolase | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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