DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Y | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Martin-Lopez, JV | - |
dc.contributor.author | Lee, R | - |
dc.contributor.author | Oh, J | - |
dc.contributor.author | Hanne, J | - |
dc.contributor.author | Fishel, R | - |
dc.contributor.author | Lee, JB | - |
dc.date.accessioned | 2016-08-26T07:36:05Z | - |
dc.date.available | 2016-08-26T07:36:05Z | - |
dc.date.created | 2016-07-22 | - |
dc.date.issued | 2016-03-22 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.other | 2016-OAK-0000036071 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29994 | - |
dc.description.abstract | Mismatch repair (MMR) is activated by evolutionarily conserved MutS homologs (MSH) and MutL homologs (MLH/PMS). MSH recognizes mismatched nucleotides and form extremely stable sliding clamps that may be bound by MLH/PMS to ultimately authorize strand-specific excision starting at a distant 3'- or 5'-DNA scission. The mechanical processes associated with a complete MMR reaction remain enigmatic. The purified human (Homo sapien or Hs) 5'-MMR excision reaction requires the HsMSH2-HsMSH6 heterodimer, the 5' -> 3' exonuclease HsEXOI, and the single-stranded binding heterotrimer HsRPA. The HsMLH1-HsPMS2 heterodimer substantially influences 5'-MMR excision in cell extracts but is not required in the purified system. Using real-time single-molecule imaging, we show that HsRPA or Escherichia coli EcSSB restricts HsEXOI excision activity on nicked or gapped DNA. HsMSH2-HsMSH6 activates HsEXOI by overcoming HsRPA/EcSSB inhibition and exploits multiple dynamic sliding clamps to increase tract length. Conversely, HsMLH1-HsPMS2 regulates tract length by controlling the number of excision complexes, providing a link to 5' MMR. | - |
dc.description.statementofresponsibility | open | - |
dc.language | English | - |
dc.publisher | National academy of sciences | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.title | Dynamic control of strand excision during human DNA mismatch repair | - |
dc.type | Article | - |
dc.contributor.college | 물리학과 | - |
dc.identifier.doi | 10.1073/PNAS.1523748113 | - |
dc.author.google | Jeon, Y., Kim, D., Martín-López, J.V., Lee, R., Oh, J., Hanne, J., Fishel, R., Lee, J.-B. | - |
dc.relation.volume | 113 | - |
dc.relation.issue | 12 | - |
dc.relation.startpage | 3281 | - |
dc.relation.lastpage | 3286 | - |
dc.contributor.id | 10171158 | - |
dc.relation.journal | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.113, no.12, pp.3281 - 3286 | - |
dc.identifier.wosid | 000372488200054 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 3286 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 3281 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 113 | - |
dc.contributor.affiliatedAuthor | Lee, JB | - |
dc.identifier.scopusid | 2-s2.0-84962240768 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 13 | - |
dc.description.scptc | 7 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | REPLICATION PROTEIN-A | - |
dc.subject.keywordPlus | EXONUCLEASE 1 | - |
dc.subject.keywordPlus | MUTS | - |
dc.subject.keywordPlus | RECONSTITUTION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | HMSH2-HMSH6 | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MSH2 | - |
dc.subject.keywordPlus | RPA | - |
dc.subject.keywordAuthor | Lynch syndrome/HNPCC | - |
dc.subject.keywordAuthor | single molecule | - |
dc.subject.keywordAuthor | MSH2-MSH6 | - |
dc.subject.keywordAuthor | MLH1-PMS2 | - |
dc.subject.keywordAuthor | EXOI | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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